[PDF] The Proteome Revisited eBook

Author : P. G. Righetti
Publisher : Elsevier
Page : 414 pages
File Size : 11,37 MB
Release : 2001-10-25
Category : Medical
ISBN : 9780444505262

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The book deals with the theory and practice of all electrophoretic steps leading to proteome analysis, i.e. isoelectric focusing (including immobilized pH gradients), sodium dodecyl sulphate electrophoresis (SADS-PAGE) and finally two-dimensional maps. It is a reasoned collection of all modern, relevant, up-to-date methodologies leading to successful fractionation, analysis and characterization of every polypeptide spot in 2-D map analysis. It includes chapters on the most sophisticated mass spectrometry developments and it helps the reader in navigating through the most important databases in proteome analysis, including step by step tours in selected sites. Yet, this book's unique strength and feature is the fact that it combines not only practice (in common with any other book on this topic) but also theory, by giving a detailed treatment on the most advanced theoretical treatments of steady-state techniques, such as isoelectric focusing and immobilized pH gradients. A lot of this theory is newly developed and presented to the public for the first time. Thus, this book should satisfy not only the needs of every day practitioners, but also the desires of the most advanced theoreticians in the field, who will surely appreciate the novel theories presented here. Also the methodological section contains several as yet unpublished protocols, correcting some of the existing ones and showing the pitfall and limitations of even well ingrained protocols in proteome analysis, which are here critically re-evaluated for the first time.

Author : Lucio Comai
Publisher : Humana
Page : 0 pages
File Size : 15,22 MB
Release : 2017-02-11
Category : Science
ISBN : 9781493967452

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This volume aims to provide protocols on a wide range of biochemical methods, analytical approaches, and bioinformatics tools developed to analyze the proteome. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Proteomics: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Author : David S. Eisenberg
Publisher :
Page : 334 pages
File Size : 19,80 MB
Release : 1995
Category : Science
ISBN : 9780120342464

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The topics covered by this volume include: protein destabilization at low temperatures; engineering the stability and function of Gene V Protein; free energy balance in protein folding; modelling protein stability as a heteropolymer collapse; stability of alpha helices; protein stability with T4 Lysozyme.

Author : Timothy Palzkill
Publisher : Springer Science & Business Media
Page : 128 pages
File Size : 29,41 MB
Release : 2002
Category : Medical
ISBN : 0792375653

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"Proteomics" is an introduction to the exciting new fieldof proteomics, an interdisciplinary science that includes biology, bioinformatics, and protein chemistry. The purpose of this book is toprovide the active researcher with an overview of the types ofquestions being addressed in proteomics studies and the technologiesused to address those questions.Key subjects covered in this book include: "Proteomics" provides a starting point for researchers who wouldlike a theoretical understanding of the new technologies in the field, and obtain a solid grasp of the fundamentals before integrating newtools into their experiments. Written with attention to detail, butwithout being overwhelmingly technical, "Proteomics" is auser-friendly guide needed by most biologists today.

Author : Mark Ptashne
Publisher : CSHL Press
Page : 172 pages
File Size : 21,3 MB
Release : 2004
Category : Medical
ISBN : 9780879697167

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The first edition of Mark Ptashne's 1986 book describing the principles of gene regulation in phage lambda became a classic in both content and form, setting a standard of clarity and precise prose that has rarely been bettered. This edition is a reprint of the original text, together with a new chapter updating the story to 2004. Among the striking new developments are recent findings on long-range interactions between proteins bound to widely separated sites on the phage genome, and a detailed description of how gene activation works.

Author : Cornelis A. M. van Gestel
Publisher : CRC Press
Page : 312 pages
File Size : 10,86 MB
Release : 2016-04-19
Category : Medical
ISBN : 1439830096

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In the last decade and a half, great progress has been made in the development of concepts and models for mixture toxicity, both in human and environmental toxicology. However, due to their different protection goals, developments have often progressed in parallel but with little integration. Arguably the first book to clearly link ecotoxicology an

Author : Nader Rifai
Publisher : Elsevier
Page : 218 pages
File Size : 26,81 MB
Release : 2018-06-26
Category : Science
ISBN : 0128160640

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Principles and Applications of Clinical Mass Spectrometry: Small Molecules, Peptides, and Pathogens is a concise resource for quick implementation of mass spectrometry methods in clinical laboratory work. Focusing on the practical use of these techniques, the first half of the book covers principles of chromatographic separations, principles and types of mass spectrometers, and sample preparation for analysis; the second half outlines the main applications of this technology within clinical laboratory settings, including determination of small molecules and peptides, as well as pathogen identification. A thorough yet succinct guide to using mass spectrometry technology in the clinical laboratory, Principles and Applications of Clinical Mass Spectrometry: Small Molecules, Peptides, and Pathogens is an essential resource for chemists, pharmaceutical and biotech researchers, certain government agencies, and standardization groups. Provides concrete examples of the main applications of mass spectrometry technology Describes current capabilities of the LC- and MS-based analytical methods Details methods for successful analytical work in the field

Author : M. Michael Gromiha
Publisher : Academic Press
Page : 349 pages
File Size : 34,33 MB
Release : 2011-04-21
Category : Science
ISBN : 0123884241

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One of the most pressing tasks in biotechnology today is to unlock the function of each of the thousands of new genes identified every day. Scientists do this by analyzing and interpreting proteins, which are considered the task force of a gene. This single source reference covers all aspects of proteins, explaining fundamentals, synthesizing the latest literature, and demonstrating the most important bioinformatics tools available today for protein analysis, interpretation and prediction. Students and researchers of biotechnology, bioinformatics, proteomics, protein engineering, biophysics, computational biology, molecular modeling, and drug design will find this a ready reference for staying current and productive in this fast evolving interdisciplinary field. Explains all aspects of proteins including sequence and structure analysis, prediction of protein structures, protein folding, protein stability, and protein interactions Presents a cohesive and accessible overview of the field, using illustrations to explain key concepts and detailed exercises for students.