[PDF] The Application Of Nuclear Magnetic Resonance To Molecules Undergoing Rapid Axial Reorientation eBook

Author : Ivana Komljenovic
Publisher :
Page : pages
File Size : 12,67 MB
Release : 2018
Category :
ISBN :

GET BOOK

The cell membrane is critical for life. The functionality of this membrane is dependent on the interactions between the many different molecules found within and connected to the cell membrane. Proteins and peptides are responsible for a variety of biological activities including signal transmission and ion movement. Membrane proteins can be associated very closely with the membrane (known as integral membrane proteins) or loosely with the membrane (peripheral proteins). The functionality of these membrane proteins depends on their structure, which can be studied using nuclear magnetic resonance (NMR). In structural studies, protons present themselves as desirable nuclei for detection, owing to their high abundance in biological systems. However, strong homonuclear dipolar interactions often lead to broad signals and obscured details. This thesis describes the study and development of NMR pulse sequences that can be used to investigate model membrane systems that include small molecules. Beginning with a leucine-rich peptide P0813, proton suppression techniques were developed using a unique gradient probe assembly. The addition of pulsed field gradients within the pulse sequences resulted in significant proton coherence suppression in a sample composed of protonated lipid and peptide. Gramicidin-A was used in the development of proton-detected correlation experiments. These experiments were further studied with Conolysin-Mt1, a natural cytolytic peptide found in sea snails. Cholesterol was employed in the study of how the nuclear Overhauser effect can be used to probe molecular structure and dynamics. Finally, the majority of the modified experiments were tested on a synthetic peptide ALGA, which was originally designed to produce well-resolved proton spectra. Throughout the studies performed on the five different small molecules, new proton detection techniques were investigated, with varying degrees of success. In addition, different sample preparations were explored to help determine how changes in sample composition can affect sample stability.

Author : Krystyna Kamienska-Trela
Publisher : Royal Society of Chemistry
Page : 499 pages
File Size : 14,23 MB
Release : 2012
Category : Medical
ISBN : 1849733732

GET BOOK

As a spectroscopic method, nuclear magnetic resonance (NMR) has seen spectacular growth, both as a technique and in its applications. Today's applications of NMR span a wide range of scientific disciplines, from physics to biology to medicine. Each volume of Nuclear Magnetic Resonance comprises a combination of annual and biennial reports which together provide comprehensive coverage of the literature on this topic. This Specialist Periodical Report reflects the growing volume of published work involving NMR techniques and applications, in particular NMR of natural macromolecules, which is covered in two reports: NMR of Proteins and Nucleic Acids and NMR of Carbohydrates, Lipids and Membranes. In his foreword to the first volume, the then editor, Professor Robin Harris announced that the series would be a discussion on the phenomena of NMR and that articles will be critical surveys of the literature. This has certainly remained the case throughout the series, and in line with its predecessors, Volume 40 aims to provide a comprehensive coverage of the relevant NMR literature. For the current volume this relates to publications appearing between June 2009 and May 2010 (the nominal period of coverage in volume 1 was July 1970 to June 1971). Compared to the previous volume there are some new members of the reporting team. Theoretical Aspects of Spin-Spin Couplings are covered by J. Jazwinski, while E. Swiezewska and J.W̤jcik provide an account of NMR of Carbohydrates, Lipids and Membranes.

Author : J. L. Atwood
Publisher : CRC Press
Page : 1002 pages
File Size : 48,88 MB
Release : 2004
Category : Science
ISBN : 9780824747244

GET BOOK

Covers the fundamentals of supramolecular chemistry; supramolecular advancements and methods in the areas of chemistry, biochemistry, biology, environmental and materials science and engineering, physics, computer science, and applied mathematics.

Author : J. Mason
Publisher : Springer Science & Business Media
Page : 642 pages
File Size : 40,74 MB
Release : 2012-12-06
Category : Science
ISBN : 1461317835

GET BOOK

With the power and range of modern pulse spectrometers the compass of NMR spec troscopy is now very large for a single book-but we have undertaken this. Our book covers the Periodic Table as multinuclear spectrometers do, and introductory chapters are devoted to the essentials of the NMR experiment and its products. Primary products are chemical shifts (including anisotropies), spin-spin coupling constants, and relaxation times; the ultimate product is a knowledge of content and constitution, dynamic as well as static. Our province is chemical and biochemical rather than physical or technical; only passing reference is made to metallic solids or unstable species, or to practical NMR spectroscopy. Our aim is depth as well as breadth, to explain the fundamental processes, whether of nuclear magnetic shielding, spin-spin coupling, relaxation, or the multiple pulse sequences that have allowed the development of high-resolution studies of solids, multidimensional NMR spectroscopy, techniques for sensitivity enhancement, and so on. This book therefore combines the functions of advanced textbook and reference book. For reasonably comprehensive coverage in a single volume we have sum marized the information in tables and charts, and included all leading references.

Author : David M. Grant
Publisher : John Wiley & Sons
Page : 888 pages
File Size : 31,97 MB
Release : 1996
Category : Medical
ISBN :

GET BOOK

The content of this volume has been added to the online reference work Encyclopedia of Magnetic Resonance. For further information see Encyclopedia of Magnetic Resonance. As a stand alone volume, Advances in NMR comprehensively highlights the rapid progress of nuclear magnetic resonance over the last five years. Features 66 articles on the latest major advances in NMR Written by over 80 internationally recognised experts With over 900 pages, illustrated extensively throughout, and an easy to read large double-columned format, Advances in NMR covers indepth articles on the latest advances in spectroscopic techniques; nuclear interactions; biochemical, physical and chemical applications. Including these outstanding articles: Double-Quantum NMR Spectroscopy of Dipolar Coupled Spins Under Fast Magic Angle Spinning (H W Spiess) Pulse Sequence Design using Rotor and Spin Symmetry ( M Levitt) Indirect Nuclear Spin-Spin Coupling Tensors (R E Wasylishen) Weakly Aligned Biomolecules in Liquid Crystals (A Bax) Multiple-Resonance, Multi-dimensional Solid-state NMR of Proteins (S J Opella) Dynamics of Hydrogen Transfer in Liquids and Solids (H Limbach) Optically Pumped NMR of Semiconductors and Two-dimensional Electron Systems (R Tycko/S E Barrett) "The list of contributors looks like a Who's Who of the subject" —The Times Higher Education Supplement

Author : Ferid Bashirov
Publisher : CRC Press
Page : 164 pages
File Size : 26,76 MB
Release : 2011-10-13
Category : Science
ISBN : 1439870837

GET BOOK

Spectroscopic Techniques and Hindered Molecular Motion presents a united, theoretical approach to studying classical local thermal motion of small molecules and molecular fragments in crystals by spectroscopic techniques. Mono- and polycrystalline case studies demonstrate performance validity. The book focuses on small molecules and molecular fragments, such as N2, HCl, CO2, CH4, H2O, NH4, BeF4, NH3, CH2, CH3, C6H6, SF6, and other symmetrical atomic formations, which exhibit local hindered motion in molecular condensed media: molecular and ionic crystals, molecular liquids, liquid crystals, polymeric solids, and biological objects. It reviews the state of studying the hindered molecular motion (HMM) phenomenon and the experimental works on the basis of the latest theoretical research. Case Studies Physical models of hindered molecular motion General solution of the stochastic problem for the hindered molecular motion in crystals Formulae of the angular autocorrelation function symmetrized on the crystallographic point symmetry groups Formulae of the spectral line shapes concerning the dielectric, infrared, Raman, nuclear magnetic relaxation, and neutron scattering spectroscopy in the presence of the hindered molecular motion Experimental probation of the theoretical outcomes Proton relaxation in three-atomic molecular fragments undergoing axial symmetry hindered motion Structural distortion in the ordered phase of crystalline ammonium chloride Organic compounds, polymers, pharmaceutical products, and biological systems consist of the molecular fragments, which possess rotational or conformational degrees of freedom or an atomic exchange within the fragments. Liquid crystals present a menagerie of substances for which orientational ordering shows special significance. Knowledge of the laws of molecular motion stimulates growing new crystals and synthesizing new substances with the desired properties. Although this book does not exhaust all varieties of scientific and engineering problems, it is a fundamental and comprehensive resource and catalyst for further HMM research.

Author : National Research Council
Publisher : National Academies Press
Page : 222 pages
File Size : 40,53 MB
Release : 2006-06-01
Category : Science
ISBN : 030916463X

GET BOOK

Scientists and engineers have long relied on the power of imaging techniques to help see objects invisible to the naked eye, and thus, to advance scientific knowledge. These experts are constantly pushing the limits of technology in pursuit of chemical imagingâ€"the ability to visualize molecular structures and chemical composition in time and space as actual events unfoldâ€"from the smallest dimension of a biological system to the widest expanse of a distant galaxy. Chemical imaging has a variety of applications for almost every facet of our daily lives, ranging from medical diagnosis and treatment to the study and design of material properties in new products. In addition to highlighting advances in chemical imaging that could have the greatest impact on critical problems in science and technology, Visualizing Chemistry reviews the current state of chemical imaging technology, identifies promising future developments and their applications, and suggests a research and educational agenda to enable breakthrough improvements.

Author : Ivano Bertini
Publisher : Elsevier
Page : 510 pages
File Size : 11,35 MB
Release : 2016-09-15
Category : Science
ISBN : 0444634487

GET BOOK

NMR of Paramagnetic Molecules: Applications to Metallobiomolecules and Models, Second Edition is a self-contained, comprehensive reference for chemists, physicists, and life scientists whose research involves analyzing paramagnetic compounds. Since the previous edition of this book was published, there have been many advancements in the field of paramagnetic NMR spectroscopy. This completely updated and expanded edition contains the latest fundamental theory and methods for mastery of this analytical technique. Users will learn how to interpret the NMR spectra of paramagnetic molecules, improve experimental techniques, and strengthen their understanding of the underlying theory and applications. Reflects all advances in the field in a completely updated new edition Presents new material on self-orientation residual dipolar couplings, solid state NMR, dynamic nuclear polarization, and paramagnetic restraints for structure calculations Includes information relevant to paramagnetic molecules, metallobiomolecules, paramagnetic compounds, and paramagnetic NMR spectroscopy Presents specific examples of paramagnetic inorganic species and experimental techniques for structure characterization