[PDF] Preparation And Characterization Of Poly Lactic Co Glycolic Acid Nanoparticles For Ex Vivo Immunomodulation eBook

Author : Prashant Kesharwani
Publisher : Elsevier
Page : 534 pages
File Size : 29,75 MB
Release : 2023-03-02
Category : Technology & Engineering
ISBN : 0323914314

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Poly(lactic-co-glycolic acid) (PLGA) Nanoparticles for Drug Delivery is a comprehensive guide to PLGA nanoparticles for targeting various diseases, covering principles, formation, characterization, applications, regulations and the latest advances. Sections introduce the fundamental aspects of PLGA nanoparticles for drug delivery, including properties, preparation methods, characterization, drug loading methods, and drug release mechanisms, along with a focus on applications. Application of PLGA nanoparticles for the treatment of cancer, inflammatory, cerebral, cardiovascular, and infectious diseases, as well as in regenerative medicine, photodynamic and photothermal therapy, and gene therapy, are all explained in detail. The final chapters explore recent advances and regulatory aspects. This book is a valuable resource for researchers and advanced students across nanomedicine, polymer science, bio-based materials, chemistry, biomedicine, biotechnology, and materials engineering, as well as for industrial scientists and R&D professionals with an interest in nanoparticles for drug delivery, pharmaceutical formulations and regulations, and development of innovative biodegradable materials. Presents the fundamentals of PLGA nanoparticles, including properties, preparation, characterization, and biofate and cellular interactions Provides in-depth coverage of a broad range of specific applications of PLGA nanoparticles across disease treatment, regenerative medicine and therapeutic areas Offers a methodical approach to PLGA nanoparticles in drug delivery that is supported by data tables, illustrative figures and flowcharts

Author : Črt Dragar
Publisher :
Page : 128 pages
File Size : 40,9 MB
Release : 2019
Category :
ISBN :

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Poly(lactic-co-glycolic) acid nanoparticles have gained enormous interest in the recent 40 years for their use in pharmaceutics, diagnostics and theranostics. They show numerous advantages, such as biodegradability and biocompatibility, ability to deliver different types of drugs, surface properties, which enable further modifications and possibility of controlled drug release. Nonetheless, poly(lactic-co-glycolic) acid nanoparticles still have some drawbacks, which hinder their transition from research into clinical practice. The aim of this study was to establish and implement protocols for preparation of poly(lactic-co-glycolic) nanoparticles for application in drug delivery. Non-functionalized (i.e., uncoated) as well as chitosan- and poly(ethylene imine) functionalized (i.e., coated) nanoparticles were prepared and characterized with regard to their particle size and particle size distribution, zeta potential, surface hydrophobicity/ hydrophilicity, morphology, solid state properties, and cellular interactions using buccal human TR 146 cells. The rheological properties of poly(lactic-co-glycolic) acid nanoparticles dispersions were also determined. Moreover, selected formulations were freeze-dried and stability studies were conducted for two months. Poly(lactic-co-glycolic) acid nanoparticles were prepared based on emulsion-diffusion-evaporation method. Stirring rates, homogenization set up and stabilizer concentration were adjusted to achieve reproducible preparation of nanoparticle formulations. Hydrodinamic particle size ranged from 230 nm to 330 nm. Chitosan-coated nanoparticles had zeta potential of ~+30 mV, poly(ethylene imine)-coated particles showed values of ~+10 mV and uncoated nanoparticles revealed a zeta potential of ~- 5 mV. All formulations showed the polydispersity index smaller than 0.25. Freeze-drying of uncoated and poly(ethylene imine)-coated nanoparticles was successful without the use of additional lyo- and/or cryo-protectants. The freeze-dried formulations were stable at 2-8 °C for at least two months. The uncoated nanoparticles showed the highest hydrophobicity, followed by the poly(ethylene imine)- and chitosan-coated ones. The positively charged nanoparticles interacted with the cell membrane, whereas the negatively charged nanoparticles were internalized by the cells in vitro. To sum up, robust protocols were successfully established that enabled the reproducible preparation of uncoated and chitosan-coated poly(lactic-co-glycolic) acid nanoparticles. However, the protocol for preparation of poly(ethylene imine)-coated poly(lactic-co-glycolic) acid nanoparticles was shown to be inefficient and should be further optimized in the future studies.

Author : Marina Dobrovolskaia
Publisher : World Scientific
Page : 721 pages
File Size : 49,37 MB
Release : 2013
Category : Medical
ISBN : 9814390259

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The Handbook of Immunological Properties of Engineered Nanomaterials provides a comprehensive overview of the current literature, methodologies, and translational and regulatory considerations in the field of nanoimmunotoxicology. The main subject is the immunological properties of engineered nanomaterials. Focus areas include interactions between engineered nanomaterials and red blood cells, platelets, endothelial cells, professional phagocytes, T cells, B cells, dendritic cells, complement and coagulation systems, and plasma proteins, with discussions on nanoparticle sterility and sterilization. Each chapter presents a broad literature review of the given focus area, describes protocols and resources available to support research in the individual focus areas, highlights challenges, and outlines unanswered questions and future directions. In addition, the Handbook includes an overview of and serves a guide to the physicochemical characterization of engineered nanomaterials essential to conducting meaningful immunological studies of nanoparticles. Regulations related to immunotoxicity testing of materials prior to their translation into the clinic are also reviewed.The Handbook is written by top experts in the field of nanomedicine, nanotechnology, and translational bionanotechnology, representing academia, government, industry, and consulting organizations, and regulatory agencies. The Handbook is designed to serve as a textbook for students, a practical guide for research laboratories, and an informational resource for scientific consultants, reviewers, and policy makers. It is written such that both experts and beginners will find the information highly useful and applicable.

Author : Gerrit Borchard
Publisher : Frontiers Media SA
Page : 241 pages
File Size : 16,82 MB
Release : 2020-12-22
Category : Science
ISBN : 288966256X

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This eBook is a collection of articles from a Frontiers Research Topic. Frontiers Research Topics are very popular trademarks of the Frontiers Journals Series: they are collections of at least ten articles, all centered on a particular subject. With their unique mix of varied contributions from Original Research to Review Articles, Frontiers Research Topics unify the most influential researchers, the latest key findings and historical advances in a hot research area! Find out more on how to host your own Frontiers Research Topic or contribute to one as an author by contacting the Frontiers Editorial Office: frontiersin.org/about/contact.

Author : Bruna Corradetti
Publisher : Springer
Page : 254 pages
File Size : 38,66 MB
Release : 2016-11-30
Category : Technology & Engineering
ISBN : 3319454331

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This book provides a comprehensive overview of the cascade of events activated in the body following the implant of biomaterials and devices. It is one of the first books to shed light on the role of the host immune response on therapeutic efficacy, and reviews the state-of-the-art for both basic science and medical applications. The text examines advantages and disadvantages of the use of synthetic versus natural biomaterials. Particular emphasis is placed on the role of biomimicry in the development of smart strategies able to modulate infiltrating immune cells, thus reducing side effects (such as acute and chronic inflammation, fibrosis and/or implant rejection) and improving the therapeutic outcome (healing, tissue restoration). Current cutting-edge approaches in tissue engineering, regenerative medicine, and nanomedicine offer the latest insights into the role immunomodulation in improving tolerance during tissue transplant in the treatment of orthopaedic, pancreatic, and hepatic diseases. "Immune Response to Implanted Materials and Devices" is intended for an audience of graduate students and professional researchers in both academia and industry interested in the development of smart strategies, which are able to exploit the self-healing properties of the body and achieve functional tissue restoration.

Author : Surendra Nimesh
Publisher : Elsevier
Page : 552 pages
File Size : 25,39 MB
Release : 2024-05-31
Category : Medical
ISBN : 0443154023

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While there have been a large number of ongoing research projects and publications on the treatment of cancer, there is a limited number of books related to nanotechnology with emphasis on polymeric nanoparticles in cancer therapy. The book, covering all of the recent research work done on this basis, enlightens researchers and students with the benefits of polymeric nanomaterials in cancer. This book provides exhaustive knowledge on polymeric nanoparticles and cancer. Provides a complete overview of the application of different polymeric nanoparticles in cancer therapeutics: from characterization of nanoparticles to in vitro and in vivo studies. Contains a detailed description of the delivery of therapeutic molecules with different polymers realized with nanomedicine in cancer therapeutics. Discusses newly developed promising polymers in cancer. Describes polymeric nanoparticle and their role in theranostics pertaining to cancer. Reviews clinical relevance of different polymeric formulations in the field of cancer

Author : Buddy D. Ratner
Publisher : Elsevier
Page : 879 pages
File Size : 48,59 MB
Release : 2004-08-18
Category : Technology & Engineering
ISBN : 008047036X

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The second edition of this bestselling title provides the most up-to-date comprehensive review of all aspects of biomaterials science by providing a balanced, insightful approach to learning biomaterials. This reference integrates a historical perspective of materials engineering principles with biological interactions of biomaterials. Also provided within are regulatory and ethical issues in addition to future directions of the field, and a state-of-the-art update of medical and biotechnological applications. All aspects of biomaterials science are thoroughly addressed, from tissue engineering to cochlear prostheses and drug delivery systems. Over 80 contributors from academia, government and industry detail the principles of cell biology, immunology, and pathology. Focus within pertains to the clinical uses of biomaterials as components in implants, devices, and artificial organs. This reference also touches upon their uses in biotechnology as well as the characterization of the physical, chemical, biochemical and surface properties of these materials. Provides comprehensive coverage of principles and applications of all classes of biomaterials Integrates concepts of biomaterials science and biological interactions with clinical science and societal issues including law, regulation, and ethics Discusses successes and failures of biomaterials applications in clinical medicine and the future directions of the field Cover the broad spectrum of biomaterial compositions including polymers, metals, ceramics, glasses, carbons, natural materials, and composites Endorsed by the Society for Biomaterials