[PDF] Peptide Analysis Protocols eBook

Author : Ben M. Dunn
Publisher : Humana
Page : 335 pages
File Size : 25,81 MB
Release : 2013-12-06
Category : Science
ISBN : 9781489940049

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As the technology base for the preparation of increasingly c- plex peptides has improved, the methods for their purification and ana- sis have also been improved and supplemented. Peptide science routinely utilizes tools and techniques that are common to organic chemistry, p- tein chemistry, biophysical chemistry, enzymology, pharmacology, and molecular biology. A fundamental understanding of each of these areas is essential for interpreting all of the data that a peptide scientist may see. The purpose of Peptide Analysis Protocols is to provide the novice with sufficient practical information necessary to begin developing useful analysis and separation skills. Understanding and developing these skills will ultimately yield a scientist with broadened knowledge and good problem-solving abilities. Although numerous books that address d- ferent specialties, such as HPLC, FAB-MS, CE, and NMR, have been written, until now no single volume has reviewed all of these techniques with a focus on "getting started" in separation and analysis of peptides. This volume will also provide those who already possess practical knowledge of the more advanced aspects of peptide science with detailed applications for each of these protocols. Because the chapters have been written by researchers active in each of the fields that they discuss, a great deal of information on and insight into solution of real problems that they have encountered is presented. Examplary results are clearly demonstrated and discussed. For more advanced investi- tions, supplementary experiments are often suggested.

Author : Ben M. Dunn
Publisher :
Page : 335 pages
File Size : 34,21 MB
Release : 1994
Category : Biochemistry
ISBN : 9781592595235

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In this second volume of a two-volume set, state-of-the-art methods are given for the analysis of peptides. Leading authorities provide reproducible protocols for peptide analysis using HPLC, capillary zone electrophoresis, mass spectrometry, UV and fluorescence spectroscopy, to name a few. The authors describe the procedures in a practical, step-by-step fashion suitable for both experts or novices. The chapters also include a helpful notes section offering troubleshooting tips, alternative procedures, details on substituting unavailable materials, and informative explanations about why certain steps are necessary. These extra measures help to guarantee the successful outcome of your experiments.; Dunn and Pennington's Peptide Analysis Protocols offers a comprehensive collection of standard, readily reproducible methods for many procedures that will enable scientists in every area of biochemical and biological research to analyze those peptides that have been synthesized in the laboratory.

Author : Gregg B. Fields
Publisher : Humana Press
Page : 342 pages
File Size : 35,85 MB
Release : 2010-11-19
Category : Science
ISBN : 9781617376375

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This book is dedicated to the characterization of peptides and their applications for the study of biochemical systems. The contributing authors are all leaders in the field of peptide research. Part I, Characterization, presents the most recent advances in select analytical techniques. Part II, Application, presents a variety of specific applications for synthetic peptides. This book is an indispensable aid in the pursuit of new directions in peptide research.

Author : John M. Walker
Publisher : Humana
Page : 512 pages
File Size : 38,6 MB
Release : 1994-04-26
Category : Medical
ISBN :

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Basic Protein and Peptide Protocols offers an excellent collection of reproducible, step-by-step laboratory methods covering three major areas: (1) the quantitation and characterization of proteins, (2) the electrophoretic and blotting procedures used in protein isolation and characterization, and (3) the analysis of protein and peptide structure. THOUSANDS of labs are already using Basic Protein and Peptide Protocols-you should be too!

Author : Marie-Isabel Aguilar
Publisher : Springer Science & Business Media
Page : 411 pages
File Size : 32,28 MB
Release : 2008-02-03
Category : Science
ISBN : 1592597424

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The introduction of high-performance liquid chromatography (HPLC) to the analysis of peptides and proteins some 25 years ago revolutionized the biological sciences by enabling the rapid and sensitive analysis of peptide and protein structure through the exquisite speed, sensitivity, and resolution that can be easily obtained. Today, HPLC in its various modes has become the pivotal technique in the characterization of peptides and proteins and currently plays a critical role in both our understanding of biological processes and in the development of peptide- and protein-based pharmaceuticals. The number of applications of HPLC in peptide and protein purification continues to expand at an extremely rapid rate. Solid-phase peptide synthesis and recombinant DNA techniques have allowed the production of large quantities of peptides and proteins that need to be highly purified. HPLC techniques are also used extensively in the isolation and characterization of novel proteins that will become increasingly important in the postgenomic age. The design of multidimensional purification schemes to achieve high levels of product purity further demonstrates the power of HPLC techniques not only in the characterization of cellular events, but also in the production of pepti- and protein-based therapeutics. HPLC continues to be at the heart of the analytical techniques with which scientists in both academia and in industry must arm themselves to be able to fully characterize the identity, purity, and potency of peptides and proteins.

Author : Michael W. Pennington
Publisher : Humana Press
Page : 321 pages
File Size : 31,79 MB
Release : 1994-11-07
Category : Science
ISBN : 9780896032736

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Peptide synthesis has emerged as one of the most powerful tools in biochemical, pharmacological, immunological, and biophysical la- ratories. Recent improvements include general solid-phase method- ogy, new protecting groups, and automated equipment. These advances have allowed the facile synthesis of increasingly more complex p- tides. Many of these new and improved methods for the synthesis of peptides and peptide-related substances have been reported in various publications, but never compiled in a convenient handbook. Like other volumes in this series, Peptide Synthesis Protocols concentrates on the practical aspects of these procedures, providing the researcher with detailed descriptions and helpful tips about potential problems. This volume is not intended to serve as a basic guide to standard Merrifie- type solid-phase strategy, but rather to provide the researcher with some of the most recent applications in the field of peptide science. A c- panion volume, Peptide Analysis Protocols, will detail methodology for the charaterization of new synthetic peptides. Development of new methods and applications has continued actively even as this volume was in preparation. Owing to the number of contributors to this volume, it was necessary to establish a cutoff for publication purposes. We feel that all of the protocols presented are timely and up-to-date. Several promising new strategies, such as allyloxycarbonyl-based syntheses, were being developed at the time this volume was in the editing stages and will be included in future editions.

Author : Paul T. Matsudaira
Publisher : Elsevier
Page : 205 pages
File Size : 40,47 MB
Release : 2012-12-02
Category : Science
ISBN : 0080924611

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Why a Second Edition? The Second Edition provides practical answers to the general question, "How can I obtain useful sequence information from my protein or peptide?" rather than the more specific question asked in the first edition, "How can I obtain the N-terminal sequence?" Important new methods include ways of dealing with blocked N termini, computer analysis of protein sequences, and the recent revolution in mass spectrometry. Mass spectrophotometric characterization of proteins and peptides N-terminal sequencing of proteins with blocked N termini Internal amino acid sequence analysis after protease digestion in-gel and on-blot Improved microscale peptide purification methods Computer analysis of protein sequences New protocols tested and refined through everyday use in authors' laboratories Updated reference chapter covering all aspects of protein microsequencing

Author : John M. Walker
Publisher : Springer Science & Business Media
Page : 759 pages
File Size : 39,5 MB
Release : 1996
Category : Electronic books
ISBN : 0896033384

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-- 145 concise, step-by-step protocols for the analysis of proteins and peptides -- Provides more than three times as many protocols than the competitors' manuals -- in the incomparable Methods in Molecular Biology "TM" style -- at an affordable price -- Combound in a large 7 x l0 inch format for easy benchtop use -- An indispensable reference for industry-based researchers, professors, and their students