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Author : OECD
Publisher : OECD Publishing
Page : 18 pages
File Size : 44,65 MB
Release : 2015-07-28
Category :
ISBN : 9264243089
The in vitro mammalian cell gene mutation test can be used to detect gene mutations induced by chemical substances. In this test, the used genetic endpoints measure mutation at hypoxanthine-guanine phosphoribosyl transferase (HPRT), and at a transgene of xanthineguanine phosphoribosyl ...
Author : OECD
Publisher : OECD Publishing
Page : 18 pages
File Size : 48,1 MB
Release : 2016-07-29
Category :
ISBN : 9264264809
The in vitro mammalian cell gene mutation test can be used to detect gene mutations induced by chemical substances. In this test, the used genetic endpoints measure mutation at hypoxanthine-guanine phosphoribosyl transferase (HPRT), and at a transgene of xanthineguanine phosphoribosyl transferase...
Author : OECD
Publisher : OECD Publishing
Page : 10 pages
File Size : 26,80 MB
Release : 1997-07-21
Category :
ISBN : 9264071326
The in vitro mammalian cell gene mutation test can be used to detect gene mutations induced by chemical substances. In the cell lines the most commonly-used genetic endpoints measure mutation at thymidine kinase (TK) and hypoxanthine-guanine ...
Author : OECD
Publisher : OECD Publishing
Page : 37 pages
File Size : 39,11 MB
Release : 2022-06-30
Category :
ISBN : 9264366067
This Test Guideline describes an in vivo erythrocyte Pig-a gene mutation assay (hereafter called the Pig-a assay) which uses an endogenous mammalian gene, the phosphatidylinositol glycan class A gene (Pig-a), as a reporter of somatic-cell gene mutation. In vivo gene mutation tests, such as the Pig-a assay, are especially relevant for assessing mutagenicity because physiological factors, such as absorption of the test chemical from the site of exposure, distribution of the test chemical throughout the test system via systemic circulation, and in vivo metabolism and DNA repair processes, all contribute to the mutagenic responses.
Author : OECD
Publisher : OECD Publishing
Page : 25 pages
File Size : 18,95 MB
Release : 2015-07-28
Category :
ISBN : 9264242244
This TG includes two distinct in vitro mammalian gene mutation assays requiring two specific tk heterozygous cells lines: L5178Y tk+/-3.7.2C cells for the mouse lymphoma assay (MLA) and TK6 tk+/- cells for the TK6 assay.
Author : Organisation de coopération et de développement économiques
Publisher :
Page : 18 pages
File Size : 28,14 MB
Release : 2015
Category :
ISBN :
The in vitro mammalian cell gene mutation test can be used to detect gene mutations induced by chemical substances. In this test, the used genetic endpoints measure mutation at hypoxanthine-guanine phosphoribosyl transferase (HPRT), and at a transgene of xanthineguanine phosphoribosyl transferase (XPRT). The HPRT and XPRT mutation tests detect different spectra of genetic events. Cells in suspension or monolayer culture are exposed to, at least four analysable concentrations of the test substance, both with and without metabolic activation, for a suitable period of time. They are subcultured to determine cytotoxicity and to allow phenotypic expression prior to mutant selection. Cytotoxicity is usually determined by measuring the relative cloning efficiency (survival) or relative total growth of the cultures after the treatment period. The treated cultures are maintained in growth medium for a sufficient period of time, characteristic of each selected locus and cell type, to allow near-optimal phenotypic expression of induced mutations. Mutant frequency is determined by seeding known numbers of cells in medium containing the selective agent to detect mutant cells, and in medium without selective agent to determine the cloning efficiency (viability). After a suitable incubation time, colonies are counted.
Author : OECD
Publisher : OECD Publishing
Page : 16 pages
File Size : 16,17 MB
Release : 2011-07-28
Category :
ISBN : 9264122818
This Test Guideline describes an in vivo assay that detects chemicals that may induce gene mutations. In this assay, transgenic rats or mice that contain multiple copies of chromosomally integrated plasmid or phage shuttle vectors are used. The ...
Author : OECD
Publisher : OECD Publishing
Page : 7 pages
File Size : 29,22 MB
Release : 1986-10-23
Category :
ISBN : 926407144X
The Test Guideline for Unscheduled DNA Synthesis (UDS) in mammalian cells in vitro describes procedures utilizing primary cultures, human lymphocytes or established cell lines, to detect DNA repair synthesis after excision and removal of a stretch ...
Author : OECD
Publisher : OECD Publishing
Page : 22 pages
File Size : 48,41 MB
Release : 2016-07-29
Category :
ISBN : 9264264647
This test identifies agents that cause structural chromosome aberrations in cultured mammalian somatic cells.
Author : OECD
Publisher : OECD Publishing
Page : 60 pages
File Size : 14,84 MB
Release : 2017-04-13
Category :
ISBN : 9264274766
Following a general update of the Genetic Toxicology TGs in 2015, the present Document was written to provide succinct and useful information to individuals unfamiliar with genetic toxicology testing, as well as experienced individuals wishing to obtain an overview of the recent changes that ...