[PDF] Non Target Chemical Analysis Using Liquid Chromatography Differential Ion Mobility And Tandem Mass Spectrometry eBook

Author : Jadwiga Lyczko
Publisher :
Page : pages
File Size : 17,3 MB
Release : 2016
Category :
ISBN :

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The analysis of labile ions that easily decompose at various stages of the analytical process is a significant challenge for any analytical method. In this thesis, a new analytical approach based on "soft" mass spectrometry and differential ion mobility was developed that enables the detection of such labile analytes in a quick and convenient fashion, without the need for extensive sample preparation and column separation. Soft mass spectrometry approach combines techniques of electrospray ionization (ESI), differential ion mobility spectroscopy (FAIMS) and mass/tandem mass spectrometry (QTOF-MS/MS). The key feature of ESI-FAIMS-QTOF-MS is the replacement of liquid chromatography by a FAIMS separation technique which eliminates chemical background during the analysis. It also improves the quality of spectral data and provides the detection of thousands of analytes down to a part-per-trillion concentration level. Most importantly, the technique offers extremely soft conditions for ion introduction and their transport through the mass spectrometer. The greatest advantage, in using the soft mass spectrometry approach is that fragile species which were beyond the scope of conventional mass spectrometry approaches can now be analyzed. This thesis demonstrates unique capabilities of the soft ESI-FAIMS-QTOF-MS technique in non-target analysis, which led to the discovery of sulphur-containing polar congeners of thiotetronic acids (TA) in drinking water from underground sources in Canada, United States and Europe. Polar TAs accumulate in underground aquifers and appear to be the most abundant class of organic compounds in groundwater but cannot be detected by conventional mass spectrometry methods due to their extremely fragile nature. Structural elucidation of TAs detected in this study was accomplished using de novo identification approach in which the elemental composition from accurate mass measurements and the manual interpretation of MS/MS data were utilized. TA derivatives were originally isolated from soil bacteria and are investigated in the pharmaceutical industry for their potent activity against a broad spectrum of pathogenic bacteria and negligible toxicity to mammals. We suspect, TA congeners characterized in this study are natural disinfection agents protecting groundwater from bacterial contamination and have minimal biological impact on humans.

Author : Kenneth W. Busch
Publisher : Elsevier
Page : 721 pages
File Size : 27,2 MB
Release : 2011-10-13
Category : Science
ISBN : 0080469280

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Chiral Analysis covers an important area of analytical chemistry of relevance to a wide variety of scientific professionals. The target audience is scientific professionals with an undergraduate background in chemistry or a related discipline, specifically organic chemists, researchers in drug discovery, pharmaceutical researchers involved with process analysis or combinatorial libraries, and graduate students in chemistry. Chapters have been written with the nonspecialist in mind so as to be self-contained. * Broad coverage - spectroscopic and separation methods covered in a single volume * Up-to-date and detailed review of the various techniques available and/or under development in this field * Contributions from leading experts in the field

Author : Alexandre A. Shvartsburg
Publisher : CRC Press
Page : 322 pages
File Size : 13,89 MB
Release : 2008-12-24
Category : Science
ISBN : 9781420051070

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Over the last decade, scientific and engineering interests have been shifting from conventional ion mobility spectrometry (IMS) to field asymmetric waveform ion mobility spectrometry (FAIMS). Differential Ion Mobility Spectrometry: Nonlinear Ion Transport and Fundamentals of FAIMS explores this new analytical technology that separates and characterizes ions by the difference between their mobility in gases at high and low electric fields. It also covers the novel topics of higher-order differential IMS and IMS with alignment of dipole direction. The book relates the fundamentals of FAIMS and other nonlinear IMS methods to the physics of gas-phase ion transport. It begins with the basics of ion diffusion and mobility in gases, covering the main attributes of conventional IMS that are relevant to all IMS approaches. Building on this foundation, the author reviews diverse high-field transport phenomena that underlie differential IMS. He discusses the conceptual implementation and first-principles optimization of FAIMS as a filtering technique, emphasizing the dependence of FAIMS performance metrics on instrumental parameters and properties of ion species. He also explores ion reactions in FAIMS caused by field heating and the effects of inhomogeneous electric field in curved FAIMS gaps. Written by an accomplished scientist in the field, this state-of-the-art book supplies the foundation to understand the new technology of nonlinear IMS methods.

Author : Alfred L. Yergey
Publisher : Springer Science & Business Media
Page : 310 pages
File Size : 30,95 MB
Release : 2013-12-14
Category : Science
ISBN : 148993605X

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This book is intended both to be an introduction to techniques and applications of liquid chromatography/mass spectrometry and to serve as a reference for future workers. When we undertook its writing, we chose not to cover the field, particularly applications, exhaustively. Rather we wished to produce a book that would be of use to people just beginning to use the technique as well as to more advanced practitioners. In this regard, we have sought to highlight techniques and applications that are of current importance, while not neglecting descriptions of approaches that may be of significance in the future. We hope that we have succeeded in this. At the same time we hope that the bibliography, with indexes classified by author and title, will make this book of value to those who may disagree with our emphasis. ACKNOWLEDGMENTS. One of us (C. G. E. ) wishes to acknowledge the en couragment of Professor J. A. McCloskey in undertaking this project. All four of us are grateful for the continuous and expert assistance of V. A. Edmonds in the preparation of the Bibliography. Alfred L. Y ergey Bethesda, Maryland Charles G. Edmonds Richland, Washington lvor A. S. Lewis London, England Marvin L. Vestal Houston, Texas v Contents 1. Introduction . . . . . . . . . . . . . . . . . . . . . . . . . 1 . . . . . . . . . . . 2. Direct Liquid Introduction Interfaces . . . . . . . . . . 5 . . . 2. 1. Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 2. 2. Operating Principles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 2. 3. Specific DLI Interfaces . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 2. 3. 1. Capillary Inlets . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 2. 3. 2. Diaphragm Interfaces . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 2. 3. 3. Nebulizing Interfaces . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Author : Giuseppe Paglia
Publisher : Humana
Page : 0 pages
File Size : 19,86 MB
Release : 2019-11-15
Category : Science
ISBN : 9781071600290

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This book focuses on ion mobility-mass spectrometry (IM-MS) and informatics approaches to improve traditional analysis of molecules by providing fundamentals and protocols for exploiting the potential of state-of-the-art IM-MS technology for the most common analytical applications. The chapters have been organized into four parts, each dealing with a particular set of IM-MS applications: 1) metabolomics and lipidomics; 2) proteomics and glycomics; 3) imaging and ambient ionization IM-MS; and 4) bioinformatic solutions for analyzing IM-MS data and deriving CCS values. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Ion Mobility-Mass Spectrometry: Methods and Protocols serves as an ideal resource for scientists delving into the technique's unprecedented analytical advantages, enabling novel qualitative and quantitative applications for the analysis of complex biological samples.

Author : G.A. Eiceman
Publisher : CRC Press
Page : 367 pages
File Size : 38,57 MB
Release : 2005-06-23
Category : Science
ISBN : 1420038974

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Key Developments for Faster, More Precise Detection Capabilities Driven by the demand for the rapid and advanced detection of explosives, chemical and biological warfare agents, and narcotics, ion mobility spectrometry (IMS) undergone significant refinements in technology, computational capabilities, and understanding of the principles of gas phase

Author : Mark A. Brown
Publisher :
Page : 320 pages
File Size : 31,65 MB
Release : 1990
Category : Science
ISBN :

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Examines the versatility of liquid chromatography/mass spectrometry and describes the LC/MS instruments now available or under development. Among the topics covered is a discussion of the applications of LC/MS to the analysis of agricultural chemicals and their metabolites, including polar pesticide metabolites and conjugates. Annotation copyrighted by Book News, Inc., Portland, OR

Author : Benjamin J. Place
Publisher :
Page : 0 pages
File Size : 33,91 MB
Release : 2021
Category : Chemistry
ISBN :

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Non-targeted analysis is the determination of the chemical composition of a complex mixture without a priori knowledge regarding the identities or quantities of the compound. These techniques can be performed in a wide variety of materials and areas of study. Methods and results between laboratories can vary widely based on the compounds of interest and user expertise, which can cause poor interlaboratory comparability of non-targeted results. To address this knowledge gap, three research grade test materials were developed to allow for the comparison of instrument methods, specifically reversed-phase liquid chromatography with positive electrospray ionization mass spectrometry. The materials were shipped to thirty-one participating laboratories to be analyzed by their own non-targeted analysis instrumental method. Of the participants, twenty-three laboratories submitted good quality datasets and could be further evaluated. Using principal components analysis, the differences and similarities of the analytical methods could be examined. Overall, there were no obvious trends or associations between the methods of different laboratories and the review of the principal components analysis revealed no notable parameters that caused significant clustering or separation.