[PDF] Molecular Tools For The Detection And Quantification Of Toxigenic Cyanobacteria eBook

Author : Rainer Kurmayer
Publisher : John Wiley & Sons
Page : 446 pages
File Size : 17,58 MB
Release : 2017-09-05
Category : Science
ISBN : 1119332109

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A guide to state-of-the-art molecular tools for monitoring and managing the toxigenicity of cyanobacteria Runaway eutrophication and climate change has made the monitoring and management of toxigenic organisms in the world’s bodies of water more urgent than ever. In order to influence public policy regarding the detection and quantification of those organisms, it is incumbent upon scientists to raise the awareness of policy makers concerning the increased occurrence of toxigenic cyanobacteria and the threats they pose. As molecular methods can handle many samples in short time and help identify toxigenic organisms, they are reliable, cost-effective tools available for tracking toxigenic cyanobacteria worldwide. This volume arms scientists with the tools they need to track toxigenicity in surface waters and food supplies and, hopefully, to develop new techniques for managing the spread of toxic cyanobacteria. This handbook offers the first comprehensive treatment of molecular tools for monitoring toxigenic cyanobacteria. Growing out of the findings of the landmark European Cooperation in Science and Technology Cyanobacteria project (CYANOCOST), it provides detailed, practical coverage of the full array of available molecular tools and protocols, from water sampling, nucleic acid extraction, and downstream analysis—including PCR and qPCR based methods—to genotyping (DGGE), diagnostic microarrays, and community characterization using next-gen sequencing techniques. Offers an overview of the latest trends in the field, while providing a foundation for understanding and applying the tools and techniques described Provides detailed coverage of the full range of molecular tools currently available, with expert guidance on the analysis and interpretation of results Includes step-by-step guidance on standard operational procedures, including molecular tests used in environmental monitoring, with individual chapters devoted to each procedure Complements the published Handbook of Cyanobacterial Monitoring and Cyanotoxin Analysis from the CyanoCOST project This handbook is an indispensable working resource for scientists, lab technicians, and water management professionals and an excellent text/reference for graduate students and supervisors who use molecular tools. It will also be of great value to environmental health and protection officials and policy makers.

Author : N. Thajuddin
Publisher : Springer Nature
Page : 504 pages
File Size : 29,77 MB
Release : 2024-01-19
Category : Science
ISBN : 9819945143

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This protocol book provides detailed procedures for the isolation of cyanobacteria, extraction, quantification, and detection of cyanotoxins. It illustrates the sampling and processing of toxin-producing cyanobacteria in water and aquatic animal samples, detection of cyanotoxins from Anabaena, Anabaenopsis, Cylindrospermopsis, Microsystis, Microcystis, Nodularia, Nostoc, Schizotrix, Lyngbya, Raphidiopsis, Oscillatoria, Planktothrix in aquatic resources. It also covers toxicity analysis by various bioassay protocols, and in vitro and insilico analysis methods. The book also reviews the methods for cyanotoxin extraction, detection, and quantification by various tools including LC-MS/MS, HPLC, NMR, PCR, and HESI-MS/MS. A separate section is dedicated to the advanced methods in Cyanotoxin analysis including the Molecular Imprinting Method (MIM), Cellular signaling biosensor, Electrochemical sensor, Nanosensors, and screening of Polyketide synthase gene. The analysis of various toxin-producing genes like sxtA and mcy is also accounted for in this book. In a nutshell, the book gives comprehensive procedures about the basics and preliminary processes that are involved in sample collection to advanced methods incorporated into the well-explored and unexplored Cyanobacterial toxin. Consequently, this manual is useful for both beginners and advanced researchers, including postgraduate students, academicians, researchers, and scientists in the field of Cyanobacterial research. ​

Author : Treyton Michael Moore
Publisher :
Page : 55 pages
File Size : 31,45 MB
Release : 2019
Category : Electronic dissertations
ISBN :

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This study investigates the use of quantitative Polymerase Chain Reaction (qPCR) for the early detection of geosmin-producing cyanobacteria. qPCR can detect geosmin-producing cyanobacteria via their DNA. I developed a qPCR assay for this study that is capable of sensitively detecting multiple strains of the geosmin-producing Nostoc genus. The developed assay showed high sensitivity, demonstrating the possibility for its use in detecting low concentrations of geosmin-producing cyanobacteria before detectible levels of geosmin have been produced and released into the water. Through further sequencing of more geosmin-producing genera and species, the methodology outlined in this research could be applied to develop the tools necessary to predict taste-and-odor events caused by geosmin-producing cyanobacteria.

Author : J. Paul Rasmussen
Publisher : American Water Works Association
Page : 160 pages
File Size : 15,21 MB
Release : 2008
Category : Bacterial pollution of water
ISBN :

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This research sought to develop and test rapid genetic methods to identify toxic cyanobacteria. The key objectives were to (1) conduct a literature review and industry questionnaire examining options for rapid genetic tests; (2) characterize and understand the genes involved in cylindrospermopsin and anatoxin production; (3) adapt conventional PCR assays to real-time PCR; (4) develop rapid field methods for DNA preparation; and (5) develop probes for toxin genes and testing in the field.

Author : G A Codd
Publisher : Elsevier
Page : 202 pages
File Size : 14,90 MB
Release : 1994-01-01
Category : Technology & Engineering
ISBN : 1845698169

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Cyanobacteria, or blue-green algae, commonly occur in a variety of water types throughout the world. A variable, but, high proportion of the cyanobacterial blooms and scums, which can develop annually in lakes, reservoirs, canals and slow-flowing rivers, contain potent toxins. Although animal poisonings and human health problems associated with the ingestion of, or contact with, cyanobacterial scums have long been recognized, a developing understanding of the health hazards posed by the toxins requires that reliable, sensitive, specific and convenient methods are available for their detection and quantification. Detection methods for cynobacterial toxins looks at the application of biological, toxicological, biochemical and physicochemical techniques in studies of cyanobacterial toxins and at experimental methods that have identified at least 50 cyanobacterial compounds toxic to vertebrates. Academics, public health bodies, environmental protection agencies and water companies are all involved in monitoring cyanobacterial levels and effects, and this book will help to ensure that their research is not duplicated, is standardized and comparable, and that optimal progress can be made in an important area that is still in its developing stages.

Author : Ingrid Chorus
Publisher : CRC Press
Page : 701 pages
File Size : 31,92 MB
Release : 2021-03-08
Category : Technology & Engineering
ISBN : 1000262049

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Cyanobacterial toxins are among the hazardous substances most widely found in water. They occur naturally, but concentrations hazardous to human health are usually due to human activity. Therefore, to protect human health, managing lakes, reservoirs and rivers to prevent cyanobacterial blooms is critical. This second edition of Toxic Cyanobacteria in Water presents the current state of knowledge on the occurrence of cyanobacteria and cyanotoxins as well as their impacts on health through water-related exposure pathways, chiefly drinking-water and recreational activity. It provides scientific and technical background information to support hazard identification, assessment and prioritisation of the risks posed by cyanotoxins, and it outlines approaches for their management at each step of the water-use system. It sets out key practical considerations for developing management strategies, implementing efficient measures and designing monitoring programmes. This enables stakeholders to evaluate whether there is a health risk from toxic cyanobacteria and to mitigate it with appropriate measures. This book is intended for those working on toxic cyanobacteria with a specific focus on public health protection. It intends to empower professionals from different disciplines to communicate and cooperate for sustainable management of toxic cyanobacteria, including public health workers, ecologists, academics, and catchment and waterbody managers. Ingrid Chorus headed the department for Drinking-Water and Swimming-Pool Hygiene at the German Environment Agency. Martin Welker is a limnologist and microbiologist, currently with bioMérieux in Lyon, France.

Author : Jussi Meriluoto
Publisher : John Wiley & Sons
Page : 576 pages
File Size : 40,31 MB
Release : 2017-01-30
Category : Science
ISBN : 1119068681

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A valuable handbook containing reviews, practical methods and standard operating procedures. A valuable and practical working handbook containing introductory and specialist content that tackles a major and growing field of environmental, microbiological and ecotoxicological monitoring and analysis Includes introductory reviews, practical analytical chapters and a comprehensive listing of almost thirty Standard Operating Procedures (SOPs) For use in the laboratory, in academic and government institutions and industrial settings Those readers will appreciate the research that validates and updates cyanotoxin monitoring and analysis plus adding to approaches for setting standard methods that can be applied worldwide. Wayne Carmichael, Analytical and Bioanalytical Chemistry (2018).

Author : Wael N. Hozzein
Publisher : BoD – Books on Demand
Page : 106 pages
File Size : 26,8 MB
Release : 2022-01-12
Category : Science
ISBN : 1839624892

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Cyanobacteria are oxygenic organisms that play crucial roles in the cycles of carbon, nitrogen, and oxygen. They are ideal model organisms for studying photosynthesis, nitrogen fixation, and other biological processes. In addition, cyanobacteria are well recognized for their potential for a variety of biotechnological applications. This book presents a comprehensive overview of this interesting and useful group of bacteria. Chapters discuss such topics as the molecular methods applied for identifying freshwater toxigenic cyanobacteria, the diverse industrial applications of cyanobacteria, the potential of cyanobacteria in wound healing, the production of a novel hemoglobin by Synechocystis sp. PCC 6803, and the diversity, distribution, and applications of cyanobacteria in the Brazilian coastline.

Author : Prashant Kumar Singh
Publisher : Academic Press
Page : 300 pages
File Size : 10,77 MB
Release : 2022-06-26
Category : Science
ISBN : 0323984614

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Expanding Horizon of Cyanobacterial Biology discusses the different aspects of cyanobacteria cyanobacterial application, providing a better understanding of cyanobacterial metabolism. Chapters deal with cyanobacteria applications and explore how to exploit cyanobacterial metabolism for industrial applications. Sections cover cyanobacterial applications for the production of nanoparticles, cyanobacterial diversity, and the characterization of different assemblages such as cyanolichens and cyanobacterial endophytes, along with their ecological, morphological and physiological aspects. In addition, bioactive compounds and their applications are explored. Increasing attention has been paid by scientists across the globe to Cyanobacteria as they are ubiquitous microbes and, undoubtedly, an important organism in terms of carbon as well as nitrogen fixation. However, the research on these organisms is limited in terms of their diversity and distribution across the globe. Provides background knowledge for researchers concerned with cyanobacterial diversity and characterization of different assemblages Describes the exploitation possibility of cyanobacterial species for human welfare Discusses the different aspects of cyanobacteria, cyanobacterial application and better understanding of cyanobacterial metabolism Deals with the exploitation of cyanobacteria and their mats for bioremediation purposes Includes cyanobacterial nanotechnology and its applications in industry and allied sectors

Author : Shailendra Pratap Singh
Publisher : CRC Press
Page : 252 pages
File Size : 44,29 MB
Release : 2024-06-14
Category : Science
ISBN : 1040029086

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This laboratory reference compiles the essential protocols used in cyanobacterial research. Cyanobacteria is a model system for biofuel production and sequestration of carbon dioxide to tackle global climate change due to increasing levels of greenhouse gases. Topics range from identification and growth parameters to biomolecule estimation and isolation. It also covers genome analysis and bioengineering of cyanobacteria for novel products. Key Features: Provides step-by-step procedures and troubleshooting tips Covers identification, growth parameters and pigment estimation methodologies Describes isolation, characterization and purification of microcystin, phycobiliproteins and scytonemin from cyanobacteria Discusses genomics, proteomics and bioengineering of cyanobacteria from novel products Explains sample preparation and visualization protocol for electron microscopy-based analysis of cyanobacteria This collection is useful to students and researchers in life sciences. It is also meant for industry experts who are involved in the production of biofuels, biofertilizers, other value-added products, and carbon sequestration using cyanobacteria.