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This is an ideal practical manual of biochemistry for MBBS students. It includes flowcharts, diagrams and colour pictures for clear visualization and understanding of the topics. Formulation of working reagents has been described along with each experiment. The manual includes viva-voce questions as well as information on biomedical waste segregations and disposal.
This book will serve as a practical manual for undergraduate students in MBBS. Related clinical concepts will also be useful in the preparation of postgraduate entrance exams. This book will serve as a practical manual for undergraduate students in MBBS. Related clinical concepts will also to useful in the preparation of Post-graduate entrance exams.
1. General General Instructions Laboratory Equipments and Procedures Buffers and pH Quality Control 2. Analysis of Urine and Cerebrospinal Fluid Normal Urine: Characteristics and Analysis Abnormal Constituents of Urine Screening of Urine for Inborn Errors of Metabolism and the Use of Paper Chromatography Composition of Cerebrospinal Fluid 3. Quantitative Experiments Principles of Colorimetry Principles of Spectrophotometer Estimation of Glucose Glucose Tolerance Test Estimation of Serum and Urine Creatinine: Creatinine Clearance Estimation of Urea Serum Proteins--Albumin: Globulin Ratio Serum Total Cholesterol Lipid Profile Serum Calcium and Phosphorus Serum Bilirubin Serum Transaminases Serum Alkaline Phosphatase Serum Uric Acid 4. Equipments And Procedures Point of Care Testing pH Meter Paper Chromatography and Thin Layer Chromatography Protein Electrophoresis Polyacrylamide Gel Electrophoresis Electrolyte Analysis Arterial Blood Gas Analyzer Enzyme-Linked Immunosorbent Assay Immunodiffusion Autoanalyzer DNA Isolation from Blood/Tissues 5. Function Tests Gastric Function Tests and Analysis of Gastric Juice Pancreatic Function Tests Liver Function Tests Analysis of Bile Renal Function Tests Thyroid Function Tests Adrenal Function Tests 6. Food and Energy Energy Content of Food and Glycemic Index Fats in Food 7. Clinical Case Studies Basis and Rationale of Biochemical Tests Done in Certain Clinical Conditions 8. Objective Structured Practical Examination Objective Structured Practical Examination 9. Reagents Reagent Preparation 10. Normal Values Normal Values Worksheet
Most lab manuals assume a high level of knowledge among biochemistry students, as well as a large amount of experience combining knowledge from separate scientific disciplines. Biochemistry in the Lab: A Manual for Undergraduates expects little more than basic chemistry. It explains procedures clearly, as well as giving a clear explanation of the theoretical reason for those steps. Key Features: Presents a comprehensive approach to modern biochemistry laboratory teaching, together with a complete experimental experience Includes chemical biology as its foundation, teaching readers experimental methods specific to the field Provides instructor experiments that are easy to prepare and execute, at comparatively low cost Supersedes existing, older texts with information that is adjusted to modern experimental biochemistry Is written by an expert in the field This textbook presents a foundational approach to modern biochemistry laboratory teaching together with a complete experimental experience, from protein purification and characterization to advanced analytical techniques. It has modules to help instructors present the techniques used in a time critical manner, as well as several modules to study protein chemistry, including gel techniques, enzymology, crystal growth, unfolding studies, and fluorescence. It proceeds from the simplest and most important techniques to the most difficult and specialized ones. It offers instructors experiments that are easy to prepare and execute, at comparatively low cost.
The present book "Laboratory Manual of Biochemistry: Methods and Techniques" is the outcome of 17 years of teaching and research experience of the authors. Biochemistry is a comparatively recent branch but the utility and variability of research work and the dazzling pace of its development has positioned this discipline in the forefront of scientific hierarchy. As Biochemistry works at a molecular level (i.e. finer than that accessed by the ultra-modern optical or phase-contrast microscopes) it embraces other disciplines also. Biochemistry has thus strengthened the integrated approach concept and solving biological riddles. Biochemical Techniques are used in all branches of biological sciences and biotechnology. Biochemical experiments are conducted in the laboratory as practical as well as for persuing research. A researcher has to refer to many journals and books before he/she could get to the working protocol for his/her experiment. This book attempts to give often-used methods in a single volume. This first edition is divided into 11 Units. Each experiment includes principle, requirements, procedure, calculation and observations. At the end of each , references for additional reading are provided. Important precautions, warnings and tips are given under the notes section. In addition, there are 12 appendices, which give minute details on basic chemistry, buffer preparations and other aspects required for the conduct of the experiments. The methods given in the book will be useful for conducting practical classes at the undergraduate and postgraduate levels in biochemistry, biotechnology, microbiology, agricultural sciences, environmental science, botany, zoology, nutrition, pharmaceutical science and other biology-related subjects. This book will be a bonanza for the research workers since it covers procedures from the classical basic biochemistry to the modern PCR techniques.
Fully revised, new edition presenting latest developments in medical biochemistry. Includes many new chapters and case reports. Previous edition published in 2006.
The seventh edition of this book is a comprehensive guide to biochemistry for medical students. Divided into six sections, the book examines in depth topics relating to chemical basics of life, metabolism, clinical and applied biochemistry, nutrition, molecular biology and hormones. New chapters have been added to this edition and each chapter includes clinical case studies to help students understand clinical relevance. A 274-page free booklet of revision exercises (9789350906378), providing essay questions, short notes, viva voce and multiple choice questions is included to help students in their exam preparation. Free online access to additional clinical cases, key concepts and an image bank is also provided. Key points Fully updated, new edition providing students with comprehensive guide to biochemistry Includes a free booklet of revision exercises and free online access Highly illustrated with nearly 1500 figures, images, tables and illustrations Previous edition published in 2010