[PDF] Label Free Monitoring Of Cells In Vitro eBook

Author : Joachim Wegener
Publisher : Springer Nature
Page : 286 pages
File Size : 17,2 MB
Release : 2019-11-18
Category : Science
ISBN : 3030324338

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This book is dedicated to label-free, non-invasive monitoring of cell-based assays and it comprises the most widely applied techniques. Each approach is described and critically evaluated by an expert in the field such that researchers get an overview on what is possible and where the limitations are. The book provides the theoretical basis for each technique as well as the most successful and exciting applications. Label-free bioanalytical techniques have been known for a long time as valuable tools to monitor adsorption processes at the solid-liquid interface in general – and biomolecular interaction analysis (BIA) in particular. The underlying concepts have been progressively transferred to the analysis of cell-based assays. The strength of these approaches is implicitly given with the name 'label-free': the readout is independent of any label, reagent or additive that contaminates the system under study and potentially affects its properties. Thus, label-free techniques provide an unbiased analytical perspective in the sense that the sample is not manipulated by additives but pure. They are commonly based on physical principles and read changes in integral physical properties of the sample like refractive index, conductivity, capacitance or elastic modulus to mention just a few. Even though it is not implied in the name, label-free approaches usually monitor the cells under study non-invasively meaning that the amplitude of the signal (e.g. electric field strength, mechanical elongation) that is used for the measurement is too low to interfere or affect. In contrast to label-based analytical techniques that are commonly restricted to a single reading at a predefined time point, label-free approaches allow for a continuous observation so that the dynamics of the biological system or reaction become accessible.

Author : Maximilian Oberleitner
Publisher : Springer
Page : 391 pages
File Size : 14,60 MB
Release : 2017-09-26
Category : Science
ISBN : 331945384X

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This thesis describes novel substrate embedded physical sensors that can be used to monitor different types of cell-based assays non-invasively and label-free. The sensors described provide integrative information of the cells under study with an adaptable time resolution (ranging from milliseconds to days). This information about the dynamic cell response to chemical, physical or biological stimuli defines a new paradigm in fundamental biomedical research. The author, Maximilian Oberleitner, describes approaches in which the cells are directly grown on different sensor surfaces (gold-film electrodes, shear wave resonators or dye-doped polymer films). This approach, with the reacting cells in particularly close proximity and contact with the sensor surface, is key to a remarkable sensitivity, opening the way for a variety of new applications. This thesis not only introduces the fundamentals of each approach, but it also describes in great detail the design principles and elucidates the boundary conditions of the new sensors.

Author : Martin J. Stoddart
Publisher : Humana Press
Page : 0 pages
File Size : 23,26 MB
Release : 2011-04-12
Category : Science
ISBN : 9781617791079

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Whether the question is one of basic cell survival, or whether it is being used to correlate cell number to some other factor such as matrix synthesis, an estimate of cell viability is universally required. In Mammalian Cell Viability: Methods and Protocols, experts in the field describe methods from the most basic which can be performed in any laboratory, to some which require specific pieces of equipment. Initially focusing on methods for monolayer and suspension cells, later chapters describe methods for determining viability within tissue sections and 3 dimensional culture systems. Finally, methods requiring highly specialized equipment are described in order to explain what is possible. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and vital tips on troubleshooting and avoiding known pitfalls. Practical and adaptable, Mammalian Cell Viability: Methods and Protocols serves as a self-contained laboratory manual useful to both experienced researchers and those new to this incredibly important and influential field.

Author : Sonia Abad Tan
Publisher :
Page : 0 pages
File Size : 17,44 MB
Release : 2019
Category :
ISBN :

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This thesis focuses on cytotoxicity examination of superparamagnetic iron oxide nanoparticles (SPIONs) using different methods, including impedance spectroscopy. Despite the significant advances in adapting various biological and chemical methods to assess in-vitro toxicity of SPIONs, less attention has been paid on the development of a high throughput label-free screening platform to study the interaction between the cells and nanoparticles. In this thesis, we have taken the first step by proposing a label-free impedimetric method for monitoring cells treated with SPIONs. This study has demonstrated the effects of SPIONs on the adhesion, growth, proliferation, and viability of neuroblastoma 2A cells using impedance spectroscopy in comparison to other standard microscopic and cell viability testing methods. Results suggest that the change in impedance of electrodes exposed to the mixture of cells and SPIONs offers a wide dynamic range suitable for monitoring the effects of SPIONs with a concentration of less than 100 g/mL.

Author : Cornelia Kasper
Publisher : Springer Nature
Page : 252 pages
File Size : 33,53 MB
Release : 2021-06-09
Category : Medical
ISBN : 3030667499

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This textbook shall introduce the students to 3D cell culture approaches and applications. An overview on existing techniques and equipment is provided and insight into various aspects and challenges that researchers need to consider and face during culture of 3D cells is given. The reader will learn the importance of physiological cell, tissue and organ models and gains important knowledge on 3D analytics. This textbook deepens selected aspects of the textbook “Cell Culture Technology”, which also is published in this series, while offering extended insight into 3D cell culture. The concept of the textbook encompasses various lectures ranging from basics in cell cultivation, tissue engineering, biomaterials and biocompatibility, in vitro test systems and regenerative medicine. The textbook addresses Master- and PhD students interested and/or working in the field of modern cell culture applications and will support the understanding of the essential strategies in 3D cell culture and waken awareness for the potentials and challenges of this application.

Author : Haian Fu
Publisher : Cambridge University Press
Page : 359 pages
File Size : 25,40 MB
Release : 2012-02-13
Category : Medical
ISBN : 0521889480

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This text covers new techniques and applications in chemical genomics for researchers, professionals and graduates in biology, biomedicine and chemistry.

Author : Nils-Petter Rudqvist
Publisher : Academic Press
Page : 554 pages
File Size : 40,9 MB
Release : 2020-01-15
Category :
ISBN : 0128186739

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Tumor Immunology and Immunotherapy - Cellular Methods Part A, Volume 631, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. New chapters include Detection of intracellular cytokine production by T cells with flow cytometry, High-throughput identification of human antigen-specific CD8+ and CD4+ T cells using soluble pMHC multimers, In vitro assays for effector T cell functions and activity of immunostimulatory antibodies, Ex vivo energetic profiling of tumor cells and T cells from mouse models and human samples, A cytofluorimetric assay to evaluate T cell polyfunctionality, and much more. Contains the authority of authors who are leaders in their field Provides a comprehensive source on new methods and research in enzymology

Author : Ping Wang
Publisher : Springer
Page : 242 pages
File Size : 34,32 MB
Release : 2016-12-01
Category : Science
ISBN : 9811016585

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This book focuses on cell- and molecule-based biosensors using micro/nano devices as transducers. After providing basic information on micro/nano cell- and molecule-based biosensors, it introduces readers to the basic structures and properties of micro/nano materials and their applications. The topics covered provide a comprehensive review of the current state of the art in micro/nano cell- and molecule-based biosensors as well as their future development trends, ensuring the book will be of great interest to the interdisciplinary community active in this area: researchers, engineers, biologists, medical scientists, and all those whose work involves related interdisciplinary research and applications. Dr. Ping Wang is a Professor in Department of Biomedical Engineering at Zhejiang University, Hangzhou, China. Dr. Chunsheng Wu is a Professor in Medical School at Xi’an Jiaotong University, Xi’an, China. Dr. Ning Hu is an Assistant researcher in Department of Biomedical Engineering at Zhejiang University and a Postdoctoral researcher in Medical School at Harvard University, Boston, USA. Dr. K. Jimmy Hsia is a Professor in Department of Biomedical Engineering at Carnegie Mellon University, Pittsburgh, USA.

Author : Mian Long
Publisher : Springer Science & Business Media
Page : 2385 pages
File Size : 32,35 MB
Release : 2013-02-11
Category : Technology & Engineering
ISBN : 3642293050

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The congress’s unique structure represents the two dimensions of technology and medicine: 13 themes on science and medical technologies intersect with five challenging main topics of medicine to create a maximum of synergy and integration of aspects on research, development and application. Each of the congress themes was chaired by two leading experts. The themes address specific topics of medicine and technology that provide multiple and excellent opportunities for exchanges.