[PDF] In Vitro Mutagenesis eBook

Author : Andrew Reeves
Publisher : Humana
Page : 0 pages
File Size : 30,11 MB
Release : 2016-10-06
Category : Medical
ISBN : 9781493964703

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In vitro mutagenesis remains a critical experimental approach for investigating gene and protein function at the cellular level. This volume provides a wide variety of updated and novel approaches for performing in vitro mutagenesis using such methods as genome editing, transposon (Tn) mutagenesis, site-directed, and random mutagenesis. In Vitro Mutagenesis: Methods and Protocols guides readers through methods for gene and genome editing, practical bioinformatics approaches for identifying mutagenesis targets, and novel site-directed and random mutagenesis approaches aimed at gaining a better understanding of protein-protein and protein-cofactor interactions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, In Vitro Mutagenesis: Methods and Protocols aims to provide a highly accessible and practical manual for current and future molecular biology researchers, from the beginner practitioner to the advanced investigator in fields such as molecular genetics, biochemistry, and biochemical and metabolic engineering.

Author : Jeff Braman
Publisher : Springer Science & Business Media
Page : 284 pages
File Size : 36,15 MB
Release : 2008-02-05
Category : Science
ISBN : 1592591949

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Hands-on researchers with proven track records describe in stepwise fashion their advanced mutagenesis techniques. The contributors focus on improvements to conventional site-directed mutagenesis, including a chapter on chemical site-directed mutagenesis, PCR-based mutagenesis and the modifications that allow high throughput mutagenesis experiments, and mutagenesis based on gene disruption (both in vitro- and in situ-based). Additional methods are provided for in vitro gene evolution; for gene disruption based on recombination, transposon, and casette mutagenesis; and for facilitating the introduction of multiple mutations. Time-tested and highly practical, the protocols in In Vitro Mutagenesis Protocols, 2nd Edition offer today's molecular biologists reliable and powerful techniques with which to illuminate the proteome.

Author : Andrew Reeves
Publisher : Methods in Molecular Biology
Page : 528 pages
File Size : 42,52 MB
Release : 2018-10-20
Category : Medical
ISBN : 9781493982110

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In vitro mutagenesis remains a critical experimental approach for investigating gene and protein function at the cellular level. This volume provides a wide variety of updated and novel approaches for performing in vitro mutagenesis using such methods as genome editing, transposon (Tn) mutagenesis, site-directed, and random mutagenesis. In Vitro Mutagenesis: Methods and Protocols guides readers through methods for gene and genome editing, practical bioinformatics approaches for identifying mutagenesis targets, and novel site-directed and random mutagenesis approaches aimed at gaining a better understanding of protein-protein and protein-cofactor interactions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, In Vitro Mutagenesis: Methods and Protocols aims to provide a highly accessible and practical manual for current and future molecular biology researchers, from the beginner practitioner to the advanced investigator in fields such as molecular genetics, biochemistry, and biochemical and metabolic engineering.

Author : S.Mohan Jain
Publisher : Springer Science & Business Media
Page : 548 pages
File Size : 23,5 MB
Release : 2007-09-18
Category : Technology & Engineering
ISBN : 1402063520

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Micropropagation has become a reliable and routine approach for large-scale rapid plant multiplication, which is based on plant cell, tissue and organ culture on well defined tissue culture media under aseptic conditions. A lot of research efforts are being made to develop and refine micropropagation methods and culture media for large-scale plant multiplication of several number of plant species. However, many forest and fruit tree species still remain recalcitrant to in vitro culture and require highly specific culture conditions for plant growth and development. The recent challenges on plant cell cycle regulation and the presented potential molecular mechanisms of recalcitrance are providing excellent background for understanding on totipotency and what is more development of micropropagation protocols. For large-scale in vitro plant production the important attributes are the quality, cost effectiveness, maintenance of genetic fidelity, and long-term storage. The need for appropriate in vitro plant regeneration methods for woody plants, including both forest and fruit trees, is still overwhelming in order to overcome problems facing micropropagation such as somaclonal variation, recalcitrant rooting, hyperhydricity, polyphenols, loss of material during hardening and quality of plant material. Moreover, micropropagation may be utilized, in basic research, in production of virus-free planting material, cryopreservation of endangered and elite woody species, applications in tree breeding and reforestation.

Author : Nitish Kumar
Publisher : CRC Press
Page : 302 pages
File Size : 24,19 MB
Release : 2024-02-23
Category : Science
ISBN : 1003834248

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Crop improvement using classically induced mutagenesis is now well standardized. Many new promising varieties of different crops have been successfully developed worldwide using both physical and chemical mutagens. Voluminous literature is now available on basic and applied aspects of mutagenesis. The mutation technique has been refined and holds the promise of generating much wider and more desirable variability than classical breeding. Recent advances in technology combined with classical mutation breeding offer new and exciting challenges for the development of new varieties. A global inventory of induced mutagenesis activities for crop improvement is requried. This book covers both basic and applied aspects of mutation and its impact on various crops: it is extremely well prepared and contains a huge volume of information accumulated using classically induced mutagenesis on different crops in different countries. Three key features: Describes the importance of induced mutation in crop plant research and its application to production Highlights new advances in the understanding of plant mutagenesis in crop improvement Contains contributions from major leaders in the field of plant mutation research This volume brings together all the important and relevant literature in the field. It provides a complete account of the mutation breeding of crops, presenting conclusions about the value of the method, its possibilities, limitations, and shortcomings, and the possible difficulties of further application in various crops. The initial chapters deal with the interactions between mutagenic treatment and plant material, such as aspects of mutagenic treatment, postirradiation behavior of shoot apices, and adventitious bud techniques. All available literature is then discussed crop by crop and critically evaluated. This will serve as an extremely comprehensive guide for researchers, teachers, students, and individuals who are interested in using induced mutagenesis as a tool for crop improvement.

Author : Jeff Braman
Publisher : Humana Press
Page : 442 pages
File Size : 47,56 MB
Release : 2011-08-24
Category : Science
ISBN : 9781607616610

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In the post-genomic era, in vitro mutagenesis has emerged as a critically important tool for establishing the functions of components of the proteome. The third edition of In Vitro Mutagenesis Protocols represents a practical toolbox containing protocols vital to advancing our understanding of the connection between nucleotide sequence and sequence function. Fully updated from the previous editions, this volume contains a variety of specialty tools successfully employed to unravel the intricacies of protein-protein interaction, protein structure-function, protein regulation of biological processes, and protein activity, as well as a novel section on mutagenesis methods for unique microbes as a guide to the generalization of mutagenesis strategies for a host of microbial systems. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, In Vitro Mutagenesis Protocols, Third Edition offers today's researchers a valuable compendium of reliable and powerful techniques with which to illuminate the proteome and its rich web of biological implications.

Author : Michael K. Trower
Publisher : Springer Science & Business Media
Page : 412 pages
File Size : 24,33 MB
Release : 1996
Category : Electronic books
ISBN :

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In In Vitro Mutagenesis Protocols leading experts from industrial and academic laboratories describe easily reproducible procedures for site-directed and random mutagenesis. Site-directed protocols include those based on strand-selection, PCR (including "splicing by overlap extension" and the "megaprimer" procedure), the ligase chain reaction, positive antibiotic selection, unique restriction site elimination, gapped heteroduplex formation, and solid-phase capture with the biotin/ strepavidin system. Many techniques can be used with virtually any double-stranded DNA plasmid. The random mutagenesis protocols include methods based on PCR, degenerate oligonucleotides, cassette mutagenesis, nested deletion mutagenesis, and a specialized E. coli mutator strain. These invaluable protocols facilitate the study of gene regulation and structure/function relationships in proteins and permit modification of DNA sequences for purposes such as vector construction.