[PDF] Fundamentals Of Light Microscopy And Electronic Imaging eBook

Author : Douglas B. Murphy
Publisher : John Wiley & Sons
Page : 564 pages
File Size : 49,25 MB
Release : 2012-11-05
Category : Medical
ISBN : 047169214X

GET BOOK

Fundamentals of Light Microscopy and Electronic Imaging, Second Edition provides a coherent introduction to the principles and applications of the integrated optical microscope system, covering both theoretical and practical considerations. It expands and updates discussions of multi-spectral imaging, intensified digital cameras, signal colocalization, and uses of objectives, and offers guidance in the selection of microscopes and electronic cameras, as well as appropriate auxiliary optical systems and fluorescent tags. The book is divided into three sections covering optical principles in diffraction and image formation, basic modes of light microscopy, and components of modern electronic imaging systems and image processing operations. Each chapter introduces relevant theory, followed by descriptions of instrument alignment and image interpretation. This revision includes new chapters on live cell imaging, measurement of protein dynamics, deconvolution microscopy, and interference microscopy. PowerPoint slides of the figures as well as other supplementary materials for instructors are available at a companion website: www.wiley.com/go/murphy/lightmicroscopy

Author : Jerome Mertz
Publisher : Cambridge University Press
Page : 475 pages
File Size : 28,9 MB
Release : 2019-08
Category : Medical
ISBN : 1108428304

GET BOOK

Presents a fully updated, self-contained textbook covering the core theory and practice of both classical and modern optical microscopy techniques.

Author : Shinya Inoue
Publisher : Springer Science & Business Media
Page : 600 pages
File Size : 13,26 MB
Release : 2013-11-11
Category : Medical
ISBN : 1475769253

GET BOOK

Ever since television became practical in the early 1950s, closed-circuit television (CCTV) in conjunction with the light microscope has provided large screen display, raised image contrast, and made the images formed by ultraviolet and infrared rays visible. With the introduction of large-scale integrated circuits in the last decade, TV equipment has improved by leaps and bounds, as has its application in microscopy. With modem CCTV, sometimes with the help of digital computers, we can distill the image from a scene that appears to be nothing but noise; capture fluorescence too dim to be seen; visualize structures far below the limit of resolution; crispen images hidden in fog; measure, count, and sort objects; and record in time-lapsed and high-speed sequences through the light microscope without great difficulty. In fact, video is becoming indispensable for harnessing the fullest capacity of the light microscope, a capacity that itself is much greater than could have been envisioned just a few years ago. The time seemed ripe then to review the basics of video, and of microscopy, and to examine how the two could best be combined to accomplish these tasks. The Marine Biological Laboratory short courses on Analytical and Quantitative Light Microscopy in Biology, Medicine, and the Materials Sciences, and the many inquiries I received on video microscopy, supported such an effort, and Kirk Jensen of Plenum Press persuaded me of its worth.

Author : David L. Spector
Publisher : CSHL Press
Page : 384 pages
File Size : 44,49 MB
Release : 2006
Category : Cytology
ISBN : 0879697512

GET BOOK

This manual contains selected material from Cells - a Laboratory Manual, as well as two chapters from Live Cell Imaging. It includes sections on microscopy, and on preparing and labelling specimens for microscopy.

Author : Dee Lawlor
Publisher : Springer
Page : 164 pages
File Size : 27,90 MB
Release : 2019-05-31
Category : Science
ISBN : 3030053938

GET BOOK

This book offers a beginner’s guide to using light microscopes. It begins with a brief introduction to the physics of optics, which will give the reader a basic grasp of the behaviors of light. In turn, each part of the microscope is explained using clear and simple English, together withdetailed photographs and diagrams. The reader will learn the function, care and correct use of each part. A troubleshooting section also helps resolve some of the most common issues encountered in light microscopy. Most people have a general idea of how to use a microscope, but many never get the full benefit, because they receive no training. With easy-to-follow steps and detailed images, this guide will help everyone achieve the best results, and be confident using their microscope. This book is intended for anyone using a light microscope, such as university students, people in lab environments, hobbyists, educators who teach science to young children, and anyone with a general interest in these valuable tools.

Author : Michael J. Dykstra
Publisher : Springer Science & Business Media
Page : 368 pages
File Size : 14,97 MB
Release : 2012-12-06
Category : Science
ISBN : 146840010X

GET BOOK

In this practical text, the author covers the fundamentals of biological electron microscopy - including fixation, instrumentation, and darkroom work - to provide an excellent introduction to the subject for the advanced undergraduate or graduate student.

Author : Partha Pratim Mondal
Publisher : Springer Science & Business Media
Page : 219 pages
File Size : 39,42 MB
Release : 2013-12-12
Category : Science
ISBN : 9400775458

GET BOOK

This book starts at an introductory level and leads reader to the most advanced topics in fluorescence imaging and super-resolution techniques that have enabled new developments such as nanobioimaging, multiphoton microscopy, nanometrology and nanosensors. The interdisciplinary subject of fluorescence microscopy and imaging requires complete knowledge of imaging optics and molecular physics. So, this book approaches the subject by introducing optical imaging concepts before going in more depth about advanced imaging systems and their applications. Additionally, molecular orbital theory is the important basis to present molecular physics and gain a complete understanding of light-matter interaction at the geometrical focus. The two disciplines have some overlap since light controls the molecular states of molecules and conversely, molecular states control the emitted light. These two mechanisms together determine essential imaging factors such as, molecular cross-section, Stoke shift, emission and absorption spectra, quantum yield, signal-to-noise ratio, Forster resonance energy transfer (FRET), fluorescence recovery after photobleaching (FRAP) and fluorescence lifetime. These factors form the basis of many fluorescence based devices. The book is organized into two parts. The first part deals with basics of imaging optics and its applications. The advanced part takes care of several imaging techniques and related instrumentation that are developed in the last decade pointing towards far-field diffraction unlimited imaging.

Author : Douglas E. Chandler
Publisher : Jones & Bartlett Publishers
Page : 468 pages
File Size : 22,31 MB
Release : 2009
Category : Medical
ISBN : 0763738743

GET BOOK

The Development Of Microscopy Revolutionized The World Of Cell And Molecular Biology As We Once Knew It And Will Continue To Play An Important Role In Future Discoveries. Bioimaging: Current Concepts In Light And Electron Microscopy Is The Optimal Text For Any Undergraduate Or Graduate Bioimaging Course, And Will Serve As An Important Reference Tool For The Research Scientist. This Unique Text Covers, In Great Depth, Both Light And Electron Microscopy, As Well As Other Structure And Imaging Techniques Like X-Ray Crystallography And Atomic Force Microscopy. Written In A User-Friendly Style And Covering A Broad Range Of Topics, Bioimaging Describes The State-Of-The-Art Technologies That Have Powered The Field To The Forefront Of Cellular And Molecular Biological Research.

Author : Gabriel Cristobal
Publisher : John Wiley & Sons
Page : 949 pages
File Size : 20,10 MB
Release : 2013-02-12
Category : Technology & Engineering
ISBN : 3527635254

GET BOOK

In recent years, Moore's law has fostered the steady growth of the field of digital image processing, though the computational complexity remains a problem for most of the digital image processing applications. In parallel, the research domain of optical image processing has matured, potentially bypassing the problems digital approaches were suffering and bringing new applications. The advancement of technology calls for applications and knowledge at the intersection of both areas but there is a clear knowledge gap between the digital signal processing and the optical processing communities. This book covers the fundamental basis of the optical and image processing techniques by integrating contributions from both optical and digital research communities to solve current application bottlenecks, and give rise to new applications and solutions. Besides focusing on joint research, it also aims at disseminating the knowledge existing in both domains. Applications covered include image restoration, medical imaging, surveillance, holography, etc... "a very good book that deserves to be on the bookshelf of a serious student or scientist working in these areas." Source: Optics and Photonics News