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This book provides the reader with an updated comprehensive view of the rapidly developing and fascinating field of fluorescence spectroscopy and microscopy. In recent years, fluorescence spectroscopy and microscopy have experienced rapid technological development, which has enabled the detection and monitoring of single molecules with high spatial and temporal resolution. Thanks to these developments, fluorescence has become an even more popular method in physical, biological and related fields. This book guides the reader through both basic and advanced fluorescence spectroscopy and microscopy approaches with a focus on their applications in membrane and protein biophysics. Each of the four parts: A - Fluorescence Spectroscopy, B - Fluorescence Microscopy, C - Applications of Fluorescence Spectroscopy and Microscopy to biological membranes and D - Applications of Fluorescence Spectroscopy to protein studies are written by experts within the field. The book is intended for both complete beginners who want to quickly orient themselves in the large number of existing fluorescent methods, as well as for advanced readers who are interested in particular methods and their proper use.
Volume 3 of this new series focuses on brandnew research and applications in biology, biophysics and other fields of life sciences. Many frontline researcher have contributed to this highly attractive and interdisciplinary volume which spans the entire field of present fluorescence spectroscopy including nanotechnology, membrane and DNA studies and fluorescence imaging in cancer research.
Reflecting the expanding field's need for reliable protocols, Fluorescence Spectroscopy and Microscopy: Methods and Protocols offers techniques from a worldwide team of experts on this versatile and vital subject. The topics covered fall into four broad categories: steady-state fluorescence spectroscopy, time-resolved fluorescence spectroscopy, fluorescent probe development, and the various sub-categories of fluorescence microscopy, such as fluorescence recovery after photobleaching (FRAP), live cell FRET imaging (FRETim), fluorescence lifetime imaging (FLIM), fluorescence fluctuation spectroscopy (FFS), and single-molecule fluorescence spectroscopy (smFS). Written as a part of the popular Methods in Molecular Biology series, chapters include the kind of unambiguous detail and key implementation advice that proves essential for successful results.
Reflecting the expanding field’s need for reliable protocols, Fluorescence Spectroscopy and Microscopy: Methods and Protocols offers techniques from a worldwide team of experts on this versatile and vital subject. The topics covered fall into four broad categories: steady-state fluorescence spectroscopy, time-resolved fluorescence spectroscopy, fluorescent probe development, and the various sub-categories of fluorescence microscopy, such as fluorescence recovery after photobleaching (FRAP), live cell FRET imaging (FRETim), fluorescence lifetime imaging (FLIM), fluorescence fluctuation spectroscopy (FFS), and single-molecule fluorescence spectroscopy (smFS). Written as a part of the popular Methods in Molecular Biology series, chapters include the kind of unambiguous detail and key implementation advice that proves essential for successful results. Comprehensive and practical, Fluorescence Spectroscopy and Microscopy: Methods and Protocols aims to guide both ‘novice’ and established scientists toward furthering their research with these invaluable techniques.
Detecting Signals at the Single Molecule Level: Pioneering Achievements in MicroscopyRecent advances have led to such remarkable improvements in fluorescence lifetime imaging microscopy's (FLIM) capacity for contrast and sensitivity that researchers can now employ it to detect signals at the single molecule level. FLIM also offers the additional be
In recent years, the introduction of the combined use of fluorescence spectroscopy and microscopy has opened up exciting new arenas of scientific investigation. The combination of spectroscopic techniques and video microscopy enables acquisition of quantitative data about dynamic processes. It allows the investigation of molecular activity as it occurs--at the level of a single, intact, living cell--and provides extraordinary detail regarding biological organization and function. This new work covers emerging technologies and their applications in a variety of disciplines, including chemistry, biology, physics, computer science, engineering, medicine, and pharmacology. These topics are presented by contributing authors who are leading--and pioneering--researchers in their fields. They offer a critical evaluation of both theoretical and practical aspects of the use of these combined technologies in a way that cuts across disciplines, promoting interactivity and dialogue among scientists in different fields. Each chapter is devoted to a new, unique, or otherwise novel application of the combination of imaging techniques and fluorescence spectroscopy. In addition to covering established methods and instrumentation, the contributors also identify potential new applications in a number of areas. Throughout, they emphasize the multifunctional and multidimensional uses of the marriage of fluorescence imaging spectroscopy and digital imaging microscopy. They show how these technologies can be used in combination to obtain distinct yet complementary information, allowing two- and three-dimensional measurements, as well as manipulations of the sample under study in real time. This book makes the field of fluorescence imaging spectroscopy and microscopy accessible to scientists in a variety of research fields. It is also an excellent text for graduate students, a reference to the latest techniques, and a source of ideas to stimulate future investigation. An exploration of ideas, methods, and applications in the exciting new area of fluorescence imaging spectroscopy and microscopy This book covers the latest in techniques and advances that have developed from the combined use of fluorescence imaging spectroscopy and video microscopy. A relatively new innovation, it has revolutionized molecular research in a wide range of disciplines. This approach allows acquisition of quantitative data regarding real-world biological processes--providing unprecedented insight into the physiology of living cells and their molecular structures and functions. Written by a diverse group of leading researchers who contribute from their respective scientific disciplines--and from their perspective at the cutting edge of the technology--this book * Combines a unified treatment and a critical evaluation of the field, presenting new ideas, methods, and instrumentation, with tips for developing new applications * Covers many new techniques in different scientific disciplines--from biology and chemistry to physics, engineering, and medicine * Emphasizes the multifunctional and the multidimensional uses of fluorescence spectroscopy and video microscopy, and shows how they can be applied in different combinations to obtain new information For biochemists, cell biologists, biophysicists, physiologists, neuroscientists, applied physicists, and materials scientists, as well as for scientists working in industrial, agricultural, medical, and pharmaceutical research--and for graduate students in these areas--this book is a novel resource of research opportunities, new ideas, and current practices in a dynamic and burgeoning new field.
This first volume in the new Springer Series on Fluorescence brings together fundamental and applied research from this highly interdisciplinary and field, ranging from chemistry and physics to biology and medicine. Special attention is given to supramolecular systems, sensor applications, confocal microscopy and protein-protein interactions. This carefully edited collection of articles is an invaluable tool for practitioners and novices.
The increased use of fluorescence techniques is greatly enhanced by the improved instrumentation pioneered by inventive scientists and now made available commercially by several high-tech companies. Moreover, the design and development of many new molecular probes with higher selectivity for specific microenvironmental properties has stimulated many new researchers to employ fluorescence techniques for solving their problems. This topic book, the second in his series, reflects this exciting scientific progress and deals, among others, with new approaches and new probes in fluorescence spectroscopy, single molecule fluorescence, applications in biomembrane and enzyme studies and imaging of living cells.
During the past two decades, there has been an increasing appreciation of the significant value that lifetime-based techniques can add to biomedical studies and applications of fluorescence. Bringing together perspectives of different research communities, Fluorescence Lifetime Spectroscopy and Imaging: Principles and Applications in Biomedical Diagnostics explores the remarkable advances in time-resolved fluorescence techniques and their role in a wide range of biological and clinical applications. Broadly accessible, the book captures the state-of-the-art of fluorescence lifetime metrology and imaging and provides current perspectives on their applications to biomedical studies of intact tissues and medical diagnosis. The text introduces these techniques within the wider context of fluorescence spectroscopy and describes basic principles underlying current instrumentation for fluorescence lifetime imaging and metrology (FLIM). It also covers the wide range of methods, including single channel (point) spectroscopy, fluorescence lifetime imaging microscopy, and single- and multi-photon excitation. Edited by pioneers in this field, with contributions from leading experts, the book includes an overview of complementary techniques that help researchers beginning FLIM research. It offers a comprehensive treatment of fundamental principles, instrumentation, analytical methods, and applications. It also provides an overview of the label-free contrast available from lifetime measurements of tissue autofluorescence and the prospects for exploiting this for clinical applications and biomedical research including drug discovery.