[PDF] Entomopathogenic Nematodes Systematics Phylogeny And Bacterial Symbionts eBook

Author : Khuong Nguyen
Publisher : BRILL
Page : 832 pages
File Size : 48,20 MB
Release : 2007-09-30
Category : Science
ISBN : 9047422392

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The volume deals with morphology, taxonomy and systematics of entomopathogenic nematodes (EPN) in the families Steinernematidae and Heterorhabditidae and bacteria associated with these nematodes. In the first part, history, taxonomic status, family and genus definitions of EPN are mentioned. In the second part, all useful aspects of morphology and methodology of EPN are mentioned with illustrations and SEM photographs and instructions on how to make the measurements, and how to use them in taxonomic work. A polychotomous key with SEM photographs and illustrations is provided. In the third part, full descriptions of all species are presented with latest information about each species. In the fourth part, molecular and phylogenetic methods for working with EPN are presented, including the latest information and instructions on how to use molecular data in taxonomic work. In the last part, bacteria associated with this group of nematodes are discussed in the context of the latest information about methodology, biology and taxon.

Author :
Publisher : BRILL
Page : 454 pages
File Size : 33,62 MB
Release : 2016-10-18
Category : Science
ISBN : 9004285342

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In Advances in Entomopathogenic Nematode Taxonomy and Phylogeny the numerous species of Steinernema and Heterorhabditis described since the previous volume, published in 2007, are evaluated and discussed. Valid species proposed from 2007-2015 are covered in detail, each taxon having an illustrated diagnostic description and additional data on molecular characterisation, distribution and biology, etc. An addendum gives brief details of species proposed during 2016. An overview of the taxonomy of the two families provides an up-to-date list of species for both genera, including new synonyms and detailed commentary on specific status where appropriate. Tabular keys to all valid species of Steinernema and Heterorhabditis assist in diagnostics. A chapter on phylogeny and phylogeography completes the book.

Author : Scott M. Peat
Publisher :
Page : 224 pages
File Size : 14,57 MB
Release : 2010
Category :
ISBN :

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Both insect parasitic/entomopathogenic nematodes and plant parasitic nematodes are of great economic importance. Insect parasitic/entomopathogenic nematodes provide an environmentally safe and effective method to control numerous insect pests worldwide. Alternatively, plant parasitic nematodes cause billions of dollars in crop loss worldwide. Because of these impacts, it is important to understand how these nematodes evolve, and, in the case of entomopathogenic nematodes, how their bacterial symbionts evolve. This dissertation contains six chapters. Chapter one is a review of DNA markers and their use in the phylogenetic systematics of entomopathogenetic and insect-parasitic nematodes as well as a review of phylogenetic, co-phylogenetic and population genetic methodologies. Chapter two characterizes positive destabilizing selection on the luxA gene of bioluminescent bacteria. Our data suggests that bacterial ecology and environmental osmolarity are likely driving the evolution of the luxA gene in bioluminescent bacteria. Chapter 3 examines relationships among bacteria within the genus Photorhabdus. Our analyses produced the most robust phylogenetic hypothesis to date for the genus Photorhabdus. Additionally, we show that glnA is particularly useful in resolving specific and intra-specific relationships poorly resolved in other studies. We conclude that P. asymbiotica is the sister group to P. luminescens and that the new strains HIT and JUN should be given a new group designation within P. asymbiotica. Chapter 4 characterizes the morphology of the head and feeding apparatus of fungal feeding and insect infective female morphs of the nematode Deladenus siricidicola using scanning electron microscopy. Results showed dramatic differences in head, face, and stylet morphology between the two D. siricidicola female morphs that were not detected in previous studies using only light microscopy. Chapter five utilizes comparative transciptomics to identify putative plant and insect parasitism genes in the nematode Deladenus siricidicola. Results from this study provide the first transcriptomic characterization for the nematode Deladenus siricidicola and for an insect parasitic member of the nematode infraorder Tylenchomorpha. Additionally, numerous plant parasitism gene homologues were discovered in both D. siricidicola libraries suggesting that this nematode has co-opted these plant parasitism genes for other functions. Chapter six utilizes a phylogenomic approach to estimate the phylogeny of the nematode infraorder Tylenchomorpha.

Author : S. Sivaramakrishnan
Publisher : Springer
Page : 176 pages
File Size : 20,32 MB
Release : 2022-05-07
Category : Science
ISBN : 9781071614471

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This book provides basic information and different protocols associated with the Entomopathogenic nematodes (EPNs) and their symbiotic bacteria. Entomopathogenic nematodes (EPNs) of the genera Steinernema and Heterorhabditis and their associated bacterial symbionts Xenorhabdus and Photorhabdus aid nematode infective juveniles (IJs) in infecting and killing their insect hosts, creating a unique tripartite complex of host-vector-symbiont interactions. Due to this insect killing capability, EPNs are used as biological control agents of economically important insect pests. They are also a model system to study host-parasite interactions. It provides a systematic approach to various nematode procedures including pathogenicity, reproduction, foraging behaviour. It gives a brief outline on historical aspects, nematode-bacterium complex, biology and chemical ecology of EPNs. It concisely describes host insect rearing, nematode sampling and storage, isolation techniques, counting, handling and staining of nematodes, characterization including morphological, molecular and ecological studies, mass production, virulence bioassay, field application and efficacy. The book also includes methods and techniques for their associated symbiotic bacteria. This book serves as a laboratory manual and assists the readers to undertake advanced research in different aspects related to nematodes. It is useful for researchers in the fields of nematology, microbiology, bacteriology and entomology.

Author : Randy Gaugler
Publisher : CABI
Page : 410 pages
File Size : 38,53 MB
Release : 2002-01-31
Category : Science
ISBN : 9780851997919

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Nematodes that are parasites of insects are no longer a laboratory curiosity. They have begun to be accepted as environmentally benign alternatives to the use of chemical insecticides, for the control of insect pests. Nematode worms are now applied as biological control agents against insectpests of numerous horticultural and agricultural crops. This book provides a comprehensive review of entomopathogenic nematology. It begins by reviewing fundamental biology and setting a taxonomic foundation for nematodes and their bacterial symbionts. Several chapters are devoted to functionalprocesses involved in parasitism and to nematode ecology. Later chapters describe technological advances and control methodologies.

Author : F. Shahina
Publisher : LAP Lambert Academic Publishing
Page : 360 pages
File Size : 27,54 MB
Release : 2013
Category :
ISBN : 9783659375132

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Bio-control is a major tactic in the integrated pest management system. Entomopathogenic nematodes have been recognized as one of the potential and most effective biocontrol agent for the control of major insect pest which cause serious damage to food and fiber crops of agricultural importance for the control of insect pest in agricultural crops like cotton and fruit trees, a wide range of chemical pesticides have been indiscriminately used since many decades. The long lasting impact of the chemical on the non target organism, development of insect resistance to chemical pesticides and its hazardous effects on the human and the environment, stimulated the interest of the scientist for an alternate control measure through the biocontrol means for destroying the insect pests as to increase the agricultural productivity. Among the insect pathogens entomopathogenic nematodes have been considered as exceptionally effective biocontrol agents having non polluting properties.

Author : George O. Poinar Jr.
Publisher : BRILL
Page : 436 pages
File Size : 13,70 MB
Release : 2011-03-05
Category : Science
ISBN : 9047428668

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Nematodes are one of the most abundant groups of invertebrates on the face of the earth. Their numbers are estimated to range from 1000 per cm2 in the sand-covered hydrogen sulphide ‘black zone’ beneath the ocean floors to 1.2 billion in a single hectare of soil. Estimates for their species diversity range from 100 000 to 10 million. The past history of nematodes is a mystery, since very few fossils have been discovered. This book establishes a solid base in palaeonematology with descriptions of 66 new fossil species and accounts of all previous fossil and subfossil nematodes from sedimentary deposits, coprolites, amber and mummies. It shows how nematode fossils can be used to establish lineages at various locations and time periods in the earth’s history and when nematodes entered into symbiotic and parasitic associations with plants and animals.

Author : Roland N. Perry
Publisher : CABI
Page : 313 pages
File Size : 36,57 MB
Release : 2020-11-26
Category : Science
ISBN : 1786391759

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Plant-parasitic and free-living nematodes are increasingly important in relation to food security, quarantine measures, ecology (including pollution studies), and research on host-parasite interactions. Being mostly microscopic, nematodes are challenging organisms for research. Techniques for Work with Plant and Soil Nematodes introduces the basic techniques for laboratory and field work with plant-parasitic and free-living soil-dwelling nematodes. Written by an international team of experts, this book is extensively illustrated, and addresses both fundamental traditional techniques and new methodologies. The book covers areas that have become more widespread over recent years, such as techniques used in diagnostic laboratories, including computerized methods to count and identify nematodes. Information on physiological assays, electron microscopy techniques and basic information on current molecular methodologies and their various applications is also included.