[PDF] Entomopathogenic Bacteria From Laboratory To Field Application eBook

Author : J.F. Charles
Publisher : Springer Science & Business Media
Page : 532 pages
File Size : 16,29 MB
Release : 2013-11-11
Category : Science
ISBN : 9401714290

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Entomopathogenic bacteria (Bacillus thuringiensis and B. sphaericus) are increasingly used as biopesticides to control larval insect populations which are either agricultural or forestry pests and to reduce those which as adults are vectors of severe human diseases. This new book, the first since 1993 to address all aspects of entomopathogenic bacteria, provides undergraduate and graduate students as well as research scientists with a complete, modern view of this important group of bacteria. The authors, chosen for their sustained contributions to the field, cover both fundamental and applied research in this area. The main topics include bacterial ecology and taxonomy, toxin diversity, activity and mode of action, regulation and environment of the genes, safety and ecotoxicology, production and field application of the bacteria, and outbreaks of resistant populations. The book concludes with the most recent data obtained on transgenic biotechnology and addresses environmental impact issues.

Author : Lawrence A. Lacey
Publisher : Springer Science & Business Media
Page : 852 pages
File Size : 11,82 MB
Release : 2007-09-27
Category : Science
ISBN : 1402059337

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This field manual is designed to provide background and instruction on a broad spectrum of techniques and their use in the evaluation of entomopathogens in the field. The second edition provides updated information and includes two additional chapters and 12 new contributors. The intended audience includes researchers, graduate students, practitioners of integrated pest management (IPM), regulators and those conducting environmental impact studies of entomopathogens.

Author : Lawrence A. Lacey
Publisher : Academic Press
Page : 441 pages
File Size : 21,69 MB
Release : 1997-02-27
Category : Technology & Engineering
ISBN : 0080535771

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Biological Techniques is a series of volumes aimed at introducing to a wide audience the latest advances in methodology. The pitfalls and problems of new techniques are given due consideration, as are those small but vital details not always explicit in the methods sections of journal papers. In recent years, most biological laboratories have been invaded by computers and a wealth of new DNA technology and this will be reflected in many of the titles appearing in the series. The books will be of value to advances researches and graduate students seeking to learn and apply new techniques, and will be useful to teachers of advanced undergraduate courses involving practical or project work. This manual describes the broad array of techniques that are used in insect pathology. It will provide biologists, insect pathologists, entomologists, and those interested in biological control, with the necessary information to work on a variety of pathogen groups. This book will be an essential laboratory reference for insect pathologists. Features include: * Step by-step instructions on how to isolate, identify, culture, bioassay and store the major groups of entomopathogens * Details of the practical knowledge needed by beginners to apply the techniques * Chapters written by an international group of experts * Discussion of safety testing of entomopathogens in mammals and also broader methods such as microscopy and molecular techniques * Provides extensive supplemental literature and recipes for media, fixatives and stains

Author : Lawrence A. Lacey
Publisher : Springer Science & Business Media
Page : 913 pages
File Size : 43,37 MB
Release : 2013-06-29
Category : Science
ISBN : 9401715475

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The 38 chapters of this Field Manual provide the tools required for planning experiments with entomopathogens and their implementation in the field. Basic tools include chapters on the theory and practice of microbial control agents, statistical design of experiments, equipment and application strategies. The major pathogen groups are covered in individual chapters (virus, bacteria, protozoa, fungi, nematodes). Subsequent chapters deal with the impact of naturally occurring and introduced exotic pathogens and inundative application of microbial control agents. The largest section of the Manual is composed of 21 chapters on the application and evaluation of entomopathogens in a wide range of agricultural, forest, domestic and aquatic habitats. Mites and slugs broaden the scope of the book. Supplementary techniques and media for follow-up laboratory studies are described. Three final chapters cover the evaluation of Bt transgenic plants, resistance to insect pathogens and strategies to manage it, and guidelines for evaluating the effects of MCAs on nontarget organisms. Readership: Researchers, graduate students, practitioners of integrated pest management, regulators, those doing environmental impact studies. The book is a stand-alone reference, but is also complementary to the laboratory-oriented Manual of Techniques in Insect Pathology and similar comprehensive texts.

Author : Amos Navon
Publisher : CABI
Page : 340 pages
File Size : 47,32 MB
Release : 2000-01-01
Category : Science
ISBN : 9780851999203

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This volume provides background theory and practical protocols for bioassays of bacteria, viruses, fungi and nematodes that can be used as biological control agents against insect pests of agricultural and medical importance.

Author : S. Sivaramakrishnan
Publisher : Springer
Page : 176 pages
File Size : 24,4 MB
Release : 2022-05-07
Category : Science
ISBN : 9781071614471

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This book provides basic information and different protocols associated with the Entomopathogenic nematodes (EPNs) and their symbiotic bacteria. Entomopathogenic nematodes (EPNs) of the genera Steinernema and Heterorhabditis and their associated bacterial symbionts Xenorhabdus and Photorhabdus aid nematode infective juveniles (IJs) in infecting and killing their insect hosts, creating a unique tripartite complex of host-vector-symbiont interactions. Due to this insect killing capability, EPNs are used as biological control agents of economically important insect pests. They are also a model system to study host-parasite interactions. It provides a systematic approach to various nematode procedures including pathogenicity, reproduction, foraging behaviour. It gives a brief outline on historical aspects, nematode-bacterium complex, biology and chemical ecology of EPNs. It concisely describes host insect rearing, nematode sampling and storage, isolation techniques, counting, handling and staining of nematodes, characterization including morphological, molecular and ecological studies, mass production, virulence bioassay, field application and efficacy. The book also includes methods and techniques for their associated symbiotic bacteria. This book serves as a laboratory manual and assists the readers to undertake advanced research in different aspects related to nematodes. It is useful for researchers in the fields of nematology, microbiology, bacteriology and entomology.

Author : S. Sivaramakrishnan
Publisher : Springer
Page : 176 pages
File Size : 44,19 MB
Release : 2021-06-08
Category : Science
ISBN : 9781071614440

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This book provides basic information and different protocols associated with the Entomopathogenic nematodes (EPNs) and their symbiotic bacteria. Entomopathogenic nematodes (EPNs) of the genera Steinernema and Heterorhabditis and their associated bacterial symbionts Xenorhabdus and Photorhabdus aid nematode infective juveniles (IJs) in infecting and killing their insect hosts, creating a unique tripartite complex of host-vector-symbiont interactions. Due to this insect killing capability, EPNs are used as biological control agents of economically important insect pests. They are also a model system to study host-parasite interactions. It provides a systematic approach to various nematode procedures including pathogenicity, reproduction, foraging behaviour. It gives a brief outline on historical aspects, nematode-bacterium complex, biology and chemical ecology of EPNs. It concisely describes host insect rearing, nematode sampling and storage, isolation techniques, counting, handling and staining of nematodes, characterization including morphological, molecular and ecological studies, mass production, virulence bioassay, field application and efficacy. The book also includes methods and techniques for their associated symbiotic bacteria. This book serves as a laboratory manual and assists the readers to undertake advanced research in different aspects related to nematodes. It is useful for researchers in the fields of nematology, microbiology, bacteriology and entomology.

Author : Maurizio Vurro
Publisher : Springer Science & Business Media
Page : 370 pages
File Size : 13,48 MB
Release : 2007-05-16
Category : Science
ISBN : 1402057997

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The main scientists working with enhancing fungal, bacterial, virus and insect biological control agents on different targets present the latest progress in overcoming the barrier of insufficient virulence. This multi-disciplinary group review their own work and that of others, and describe the approaches being used, the successes and the barriers yet to be overcome. There is no up-to-date equivalent work describing biocontrol, let alone enhanced biocontrol.

Author : Juan A. Morales-Ramos
Publisher : Academic Press
Page : 642 pages
File Size : 29,67 MB
Release : 2022-09-20
Category : Science
ISBN : 0128221488

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Mass Production of Beneficial Organisms: Invertebrates and Entomopathogens, Second Edition explores the latest advancements and technologies for large-scale rearing and manipulation of natural enemies while presenting ways of improving success rate, predictability of biological control procedures, and demonstrating their safe and effective use. Organized into three sections, Parasitoids and Predators, Pathogens, and Invertebrates for Other Applications, this second edition contains important new information on production technology of predatory mites and hymenopteran parasitoids for biological control, application of insects in the food industry and production methods of insects for feed and food, and production of bumble bees for pollination.Beneficial organisms include not only insect predators and parasitoids, but also mite predators, nematodes, fungi, bacteria and viruses. In the past two decades, tremendous advances have been achieved in developing technology for producing these organisms. Despite that and the globally growing research and interest in biological control and biotechnology applications, commercialization of these technologies is still in progress. This is an essential reference and teaching tool for researchers in developed and developing countries working to produce “natural enemies in biological control and integrated pest management programs. Highlights the most advanced and current techniques for mass production of beneficial organisms and methods of evaluation and quality assessment Presents methods for developing artificial diets and reviews the evaluation and assurance of the quality of mass-produced arthropods Provides an outlook of the growing industry of insects as food and feed and describes methods for mass producing the most important insect species used as animal food and food ingredients

Author : Yoshinori Tanada
Publisher : Academic Press
Page : 681 pages
File Size : 18,76 MB
Release : 2012-12-02
Category : Technology & Engineering
ISBN : 0080926258

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Insect Pathology is designed for a broad spectrum of readers. Is should be useful to students, lecturers, and researchers requiring information about the principles in insect pathology and the biology of pathogens. It should serve as a resource for specialists to learn about other insect pathogen systems, for generalists to become aware of advances in insect pathology, and for scientists and students, beginning or otherwise, interested in learning about insect pathology. This book was originally intended to update the 1949 test by E. A. Steinhaus entitled Principles of Insect Pathology. The purpose for this book was twofold: To serve (1) as a text for an insect pathology and/or biological control class and (2) as a comprehensive reference source. Because this book summarizes much of the available information, its usefulness as a textbook for an insect pathology class is apparent. Although the literature citations are extensive, they are far from complete. The literature in insect pathology is voluminous and for the past decade has been expanding at an almost exponential rate. A complete review of the literature is beyond the scope of the book, and an omission of a reference does not preclude its importance. Our citations, however, should serve as a good starting point for those who wish to obtain further information. We have attempted to cover equally all subdisciplines, but shortcomings are unavoidable. For these, we take full responsibility.