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Author : Reiner Westermeier
Publisher : John Wiley & Sons
Page : 470 pages
File Size : 40,43 MB
Release : 2016-05-16
Category : Science
ISBN : 3527338802
"Electrophoresis in Practice" ist seit mehr als zwei Jahrzehnten das Standardwerk in der Elektrophorese. Die 5. Auflage wurde sorgfältig überarbeitet und beinhaltet nun ein erweitertes Kapitel zu Mikromethoden und der chipgebundenen Elektrophorese.
Author : Reiner Westermeier
Publisher : Wiley-Blackwell
Page : 364 pages
File Size : 49,36 MB
Release : 1997-05-05
Category : Science
ISBN :
Electrophoresis; Isotachophoresis; Bloting; Intrumentation; Interpretation of electropherograms; Equipment for section II; Methods; Page of dyes; Agarose and immunoelectrophoresis; Tiration curve analysis; Native PAGE in amphoteric buffers; Agarose IEF; PAGIEF in rehydrated gels; SDS-polyacrylamide electrophoresis; Semi-dry blotting of proteins; IEE in immobilized pH gradients; High resolution 2D eletrophoresis; PAGE of double stranded DNA; Native PAGE of single stranded DNA; Denaturing gradient gel electrophoresis; Denaturing PAGE of DNA; Vertical PAGE; A trouble-shooting guide.
Author : Reiner Westermeier
Publisher : John Wiley & Sons
Page : 458 pages
File Size : 26,80 MB
Release : 2016-03-16
Category : Science
ISBN : 3527695168
This fifth edition of the successful, long-selling classic has been completely revised and expanded, omitting some topics on obsolete DNA electrophoresis, but now with a completely new section on electrophoretic micro-methods and on-the-chip electrophoresis. The text is geared towards advanced students and professionals and contains extended background sections, protocols and a trouble-shooting section. It is now also backed by a supplementary website providing all the figures for teaching purposes, as well as a selection of animated figures tested in many workshops to explain the underlying principles of the different electrophoretic methods.
Author : Reinhard Kuhn
Publisher : Springer Science & Business Media
Page : 381 pages
File Size : 23,66 MB
Release : 2013-03-07
Category : Science
ISBN : 364278058X
Capillary electrophoresis (CE) is a brand-new analytical me- thod with the capability of solving many analytical separa- tion problems very fast and economically. This method gives new information about the investigated substances which can- not easily be obtained by other means. CE has become an established method only recently, but will be implemented in almost every analytical laboratory in industry, service units and academia in the near future. The most important fields of CE application are pharmaceutical and biochemical research and quality control. The authors have exhaustive practical experience in the application of CE methods in the pharmaceutical industry and provide the reader with a comprehensive treatment of this method. The main focus is on how to solve problems when applying CE in the laboratory. Physico-chemical theory is only dealt with in depth when necessary to understand the underlying separation mechanisms in order to solve your problems at the analytical bench. An addendum includes tables on the preparation of buffers and recommended further reading.
Author : Patrick Camilleri
Publisher : CRC Press
Page : 586 pages
File Size : 31,20 MB
Release : 1997-11-25
Category : Medical
ISBN : 9780849391279
The importance of capillary electrophoresis (CE) as an analytical tool has increased dramatically over the last ten years. It has changed from being an exploratory technique, mainly of academic interest, to one that is applied to solve "real" analytical problems. CE is easily adapted to its various modes of operation, often requiring little more than a change of the buffer solution, and is quickly becoming the preferred technique when analyzing minute amounts of available material. Featuring new chapters on CE analysis of inorganic ions and carbohydrates, the new edition of Capillary Electrophoresis not only presents this method as an academic tool, but also provides applications for solving "real-world" analytical problems. This updated Second Edition reflects the increasing use of CE over the last 10 years, how it is being applied, and the basic theoretical aspects of the separation and detection methodology of CE. Capillary Electrophoresis: Theory and Practice will appeal to students and professionals of analytical chemistry, physical chemistry, biochemistry, and biotechnology and includes suitable experiments designed to be attempted by university or college students, or anyone else wishing to familiarize themselves with CE.
Author : Robert Weinberger
Publisher : Academic Press
Page : 488 pages
File Size : 10,61 MB
Release : 2000-02-16
Category : Science
ISBN :
Capillary zone electrophoresis : basic concepts -- Capillary zone electrophoresis : methods development -- Capillary zone electrophoresis : secondary equilibrium, micelles, cyclodextrins, and related reagents -- Capillary isoelectric focusing -- Size separations in capillary gels and polymer networks -- Capillary electrochromatography -- Injection -- Detection -- Putting it all together.
Author : Reiner Westermeier
Publisher : John Wiley & Sons
Page : 316 pages
File Size : 43,89 MB
Release : 1993
Category : Medical
ISBN :
Author : Bruce Birren
Publisher : Academic Press
Page : 272 pages
File Size : 13,30 MB
Release : 2012-12-02
Category : Science
ISBN : 0323137628
Pulsed Field Gel Electrophoresis: A Practical Guide is the first laboratory manual to describe the theory and practice of this technique. Based on the authors' experience developing pulsed field gel instruments and teaching procedures, this book provides everything a researcher or student needs to know in order to understand and carry out pulsed field gel experiments. Clear, well-tested protocols assume only that users have a basic familiarity with molecular biology. Thorough coverage of useful data, theory, and applications ensures that this book is also a lasting resource for more advanced practitioners of pulsed field gels. Reviews all types of pulsed field gel electrophoresis Describes all commercially available systems and summarizes advantages and limitations of each Includes step-by-step protocols for sample preparation and analysis Illustrated with photographs that depict How to run gels: What the results should look like What they look like when they go wrong Covers applications to a wide range of organisms Includes bibliography of over 900 publications and cross-referenced by topic, application, and organism
Author : Paul D. Grossman
Publisher : Academic Press
Page : 369 pages
File Size : 28,97 MB
Release : 2012-12-02
Category : Science
ISBN : 0323138195
This book is designed to be a practical guide, used by wide audience, including those new to CE, those more experienced, routine users, those interested in technology development, and those involved with applications research. References have been emphasized to allow the reader to explore the detailed specifics and theoretical foundations. This book draws together the rapidly evolving, diverse, and multidisciplinary subject of capillary electrophoresis (CE). It is designed as a practical guide to be used by a wide audience, including those new to CE as well as more experienced users. This volume presents the capabilities, limitations, potentials, and future challenges facing each area of CE. Key aspects of this technique, such as high resolution capability, full automation, high speed separations, quantification of nanoliter sample volumes, and simultaneous multiple detection capabilities are presented in a concise and logical fashion. This book is designed to help you make the most of your CE separations, and includes comprehensive information on:Electroosmosis, separation efficiency, and Joule heatingDetection methodsIn-depth discussion of the separation principles and capabilities of the major modes of CESieving gel electrophoresisIsoelectric focusingFree solution CEMicellar electrokinetic capillary chromatographyEntangled polymer matrix-based separationDetailed treatment of the application of CE to a wide range of molecules, supplemented with extensive "hands-on" illustrations
Author : Reiner Westermeier
Publisher : Wiley-Blackwell
Page : 278 pages
File Size : 27,13 MB
Release : 1993-07-15
Category : Science
ISBN : 9783527300129
A comprehensive compilation of methods for all variations of modern electrophoresis. In-depth presentation of the physical-chemical background is followed by numerous practical tips based on application-oriented examples. Both newcomers and experts will appreciate the detailed instructions and the carefully worked-out problems that characterize this book. Reiner Westermeier has become well-known in the field of electrophoresis through his numerous publications, seminars and lectures.