[PDF] Carbohydrate Analysis By Modern Liquid Phase Separation Techniques eBook

Author : Ziad El Rassi
Publisher : Elsevier
Page : 956 pages
File Size : 39,86 MB
Release : 2021-08-15
Category : Science
ISBN : 0128245123

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Carbohydrate Analysis by Modern Liquid Phase Separation Techniques, Second Edition, presents readers with the various principles of modern liquid phase separation techniques and their contributions to the analysis of complex carbohydrates and glycoconjugates. In a selection of all-new chapters, this fully updated volume covers each technique in detail. The book aims to help analysts solve any of the many practical problems they may face in tackling the analysis of carbohydrates. In addition, it addresses current difficulties that must be resolved in carbohydrate research, thus inspiring further important technological developments to meet these challenges. This is an essential resource for anyone seeking a broad view of the science of carbohydrates and separation techniques. Covers the basic principles of modern liquid phase separation techniques, along with their applications Compiles up-to-date information on the field of carbohydrate analysis, along with updates on separation science Focuses on problems currently faced in carbohydrate analysis and the solutions necessary for further progress

Author : Z. El Rassi
Publisher : Elsevier
Page : 693 pages
File Size : 48,64 MB
Release : 1994-11-11
Category : Science
ISBN : 008085866X

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Carbohydrates and glycoconjugates play an important role in several life processes. The wide variety of carbohydrate species and their inherent polydispersity and heterogeneity require separation techniques of high resolving power and high selectivity such as high performance liquid chromatography (HPLC) and capillary electrophoresis (HPCE). In the last decade HPLC, and recently HPCE methods have been developed for the high resolution and reproducible quantitation of carbohydrates. Despite the importance of these two column separation technologies in the area of carbohydrates, no previous book describes specialized methods for the separation, purification and detection of carbohydrates and glycoconjugates by HPLC and HPCE. Therefore, the objective of the present book is to provide a comprehensive review of carbohydrate analysis by HPLC and HPCE by covering analytical and preparative separation techniques for all classes of carbohydrates including mono- and disaccharides; linear and cyclic oligosaccharides; branched heterooligosaccharides (e.g., glycans, plant-derived oligosaccharides); glycoconjugates (e.g., glycolipids, glycoproteins); carbohydrates in food and beverage; compositional carbohydrates of polysaccharides; carbohydrates in biomass degradation; etc. The book will be of interest to a wide audience, including analytical chemists and biochemists, carbohydrate, glycoprotein and glycolipid chemists, molecular biologists, biotechnologists, etc. It will also be a useful reference work for both the experienced analyst and the newcomer as well as for users of HPLC and HPCE, graduates and postdoctoral students.

Author : Ziad El Rassi
Publisher : Elsevier
Page : 1255 pages
File Size : 35,38 MB
Release : 2002-10-31
Category : Science
ISBN : 008052849X

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This book is an updated and expanded edition of Carbohydrate Analysis, High Performance Liquid Chromatography and Capillary Electrophoresis and is concerned with the analysis of carbohydrates by modern chromatography and electrophoresis including analytical and preparative high performance liquid chromatography (HPLC), thin layer chromatography (TLC), field flow fractionation (FFF), capillary electrophoresis (CE), capillary electrochromatography (CEC), polyacrylamide gel electrophoresis (PAGE), gas chromatography (GC) and supercritical fluid chromatography (SFC). Thirty-one chapters cover: various modes of HPLC, CE, CEC, FFF, GC and SFC that are currently applied to the analysis of carbohydrates; discussions on analytical and preparative separations; descriptions of the principles of detection and quantitative determination of carbohydrates by the various separation techniques; reviews of sample preparations; and information on important applications. Furthermore, the book describes in detail the different direct and indirect detection methods that have been introduced for the sensitive detection of carbohydrates. This title is useful for a wide audience including separation scientists; analytical chemists and biochemists; carbohydrate chemists; glycoprotein and glycolipid chemists; molecular biologists; and biotechnologists. The book is also a useful reference for both the experienced analyst and the newcomer and for users of modern chromatography and electrophoresis. · Contains 31 chapters covering all aspects of carbohydrate analysis by modern chromatography and electrophoresis· Each chapter discusses the basic principles, advantages and limitations, and applications of the particular detection technique· Useful reference for both the experienced analyst and the newcomer

Author : Jonathan Blake Thacker
Publisher :
Page : 116 pages
File Size : 37,81 MB
Release : 2019
Category : Bioinformatics
ISBN :

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Carbohydrate analysis has many important implications in health, consumer products, and industrial processes. Among many possible analytical techniques for their analysis, mass spectrometry (MS) is an attractive approach, offering high sensitivity and specificity. In particular, electrospray ionization (ESI)-MS affords several advantages over other ionization techniques: the ability for direct coupling with upstream chemical separation techniques, its suitability for quantitation, and its compatibility with relatively inexpensive quadrupole mass analyzers for the analysis of high molecular weight compounds. However, the sensitivity of ESI-MS towards carbohydrates tends to be much less than for other classes of compounds, such as peptides. This is due to their lack of easily protonable or deprotonable chemical groups as well as their high hydrophilicities. Increasing an analyte's sensitivity in ESI-MS can be achieved by optimizing detection strategies and solvent parameters. Flow injection analysis-ESI-tandem MS (MS/MS) was used to investigate the influence of solvent parameters on the signal intensity of glucose. Solvent parameters explored included the organic solvent and its ratio to water, the additive and its concentration, and solution pH. The use of ammonium trifluoroacetate in 80:20 methanol: water in the positive ionization mode resulted in the highest signal intensities. It was also found that acetonitrile suppressed ionization in the positive ionization mode. The effects of solvent parameters on the relationship between response factors and concentrations was also studied for glucose, sucrose, and raffinose using a continuous stirred tank reactor coupled to ESI-MS/MS. This technique is able to generate a continuum of response factors across a wide concentration range from a single injection. The profiles and magnitudes of the response factor vs. concentration profiles varied widely, and were dependent upon the ionic species monitored and the solvent parameters used. Monitoring acetate, chloride, and formate adducts resulted in the best linearities, useful for quantitative analysis. Lastly, liquid chromatography-ESI-MS/MS was used to determine the concentrations of fructose, glucose, and sucrose in hard cider and apple juice samples. The method was validated with respect to selectivity, linearity, the limit of detection, the limit of quantitation, accuracy, and precision. Notably, a correlation was found between the fructose and glucose concentrations in hard cider samples that did not contain detectable amounts of sucrose (n=9; R2=0.98).

Author : Christopher J. Biermann
Publisher : CRC Press
Page : 306 pages
File Size : 48,34 MB
Release : 1988-10-31
Category : Science
ISBN : 9780849368516

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This textbook is a comprehensive guide to analysis of carbohy-drates by gas-liquid chromatography and mass spectrometry. In addition to explaining the facets of carbohydrate analysis and their relation to each other, the text also contains in-depth reference in-formation useful to practitioners in the field. Improvements in car-bohydrate analyses methodology during the past six years are also highlighted. This extensively illustrated text provides excellent data for those in carbohydrate, agriculture, and food chemistry.

Author : Garima Awasthi
Publisher : CRC Press
Page : 282 pages
File Size : 12,95 MB
Release : 2024-02-06
Category : Technology & Engineering
ISBN : 1000906817

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Arsenic has been said to be one of the world’s most toxic elements, infiltrating the food chain and finding its way into foods. Studies have recently detected high levels of arsenic in rice, which is a major concern since rice is a staple food for a large part of the world’s population. This new volume addresses this problem, covering arsenic contamination in rice and offering effective mitigation strategies.

Author : Christopher J. Biermann
Publisher : CRC Press
Page : 304 pages
File Size : 18,7 MB
Release : 2021-12-17
Category : Science
ISBN : 1000098818

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This textbook is a comprehensive guide to analysis of carbohy-drates by gas-liquid chromatography and mass spectrometry. In addition to explaining the facets of carbohydrate analysis and their relation to each other, the text also contains in-depth reference in-formation useful to practitioners in the field. Improvements in car-bohydrate analyses methodology during the past six years are also highlighted. This extensively illustrated text provides excellent data for those in carbohydrate, agriculture, and food chemistry.

Author : Salvatore Fanali
Publisher : Elsevier
Page : 1086 pages
File Size : 15,66 MB
Release : 2023-04-20
Category : Science
ISBN : 0323983006

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Liquid Chromatography: Applications, Third Edition delivers a single source of authoritative information on all aspects of the practice of modern liquid chromatography. The text gives those working in academia and industry the opportunity to learn, refresh, and deepen their understanding of the field by covering basic and advanced theoretical concepts, recognition mechanisms, conventional and advanced instrumentation, method development, data analysis, and more. This third edition addresses new developments in the field with updated chapters from expert researchers. The book is a valuable reference for research scientists, teachers, university students, industry professionals in research and development, and quality control managers. Emphasizes the integration of chromatographic methods and sample preparation Provides important data related to complex matrices, sample preparation, and data handling Covers the most interesting and valuable applications in different fields, e.g., proteomic, metabolomics, foodomics, pollutants and contaminants, and drug analysis (forensic, toxicological, pharmaceutical, biomedical) Offers comprehensive updates to all chapters Adds new chapters on selection of liquid chromatographic mode, proteomics, doping analysis, analysis of microplastics, and analysis of pharmaceutically and biologically relevant isoforms

Author :
Publisher : Elsevier
Page : 200 pages
File Size : 13,96 MB
Release : 2022-05-25
Category : Science
ISBN : 0444643486

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ICP-MS and Trace Element Analysis as Tools for Better Understanding Medical Conditions, Volume 97 discusses trace elements and how they play an important role in biological functions and metabolism in the human body. Chapters cover Biomedical analysis by ICP-MS: A focus on single cell, Advanced statistical tools and machine learning applied to trace element analysis associated with medical conditions, ICP-MS as a tool to understand trace element homeostasis in neurological disorders, High-precision isotopic analysis of essential mineral elements – possibilities for medical diagnosis and prognosis, Exploring ICP-MS as a versatile technique: From imaging to chemical speciation, and more. Discusses the latest diagnosis/pathologies using ICP-MS analysis Covers metals, isotopes and metalorganic species for medical conditions Includes ICP-MS-based techniques