[PDF] Basic Concepts In Clinical Biochemistry A Practical Guide eBook
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This book is a practical guidebook in biochemistry, for medical as well as life sciences' students. The book covers reference values, sample collection procedure and detailed protocol to perform experiments. Each experiment starts with a brief introduction of the protocol, followed by specimen requirements and procedure. The procedures are presented in a very lucid manner and discuss details of calculations and clinical interpretations,The book is divided into 29 chapters, It offers references, general guidelines and abbreviations and provides principles and procedures of clinical biochemistry tests, along with their diagnostic importance.
Finding a simple and step-by-step procedure to conduct clinical biochemistry-related analyses is a real challenge for many undergraduate, graduate students, researchers and technicians in universities and laboratories. Moreover, understanding the theory of the experiment, which is not provided in some currently available manuals, is a useful and essential requirement in the experiment for successful performance, accuracy and acceptable results. The book contains 14 chapters. The first three chapters describe essential clinical aspects in laboratory such as specimens used for clinical chemistry analysis and sample collecting methods with common sampling errors. In addition, the fundamentals and laboratory techniques commonly used for sample analysis such as centrifugation, electrophoresis, photometry, fluorometry, and chromatography are also covered in one separate chapter. The later chapters discuss the biologic basics of liver, kidney and heart diseases and the common enzymes measured to assess the function of these organs. Moreover, properties, diagnosis and analysis of vital minerals disorders such as iron, calcium, phosphate, zinc and magnesium are discussed in five different chapters. Hematological disorders related to nutrition and some case histories and comments are added in order to help students to analyze and interpret the lab results in proper way. The book also has a separate chapter with lot of case studies and their solutions for better understanding. This book will be a useful reference for new students, non-native English medicine and life science students as it relies on figures and diagrams that explain the concepts and diagnosis of diseases in a simple way. Therefore, this quick guide aims to provide and develop the basic practical skills in the users with simple steps to follow along with the theoretical explanation for better understanding. It is expected that this quick handbook will provide good tools and useful guidelines for the students and researchers as well.
This core textbook helps medical students bridge the gap between biochemistry, physiology, and clinical care. The strength of Mark's Basic Medical Biochemistry is that it starts with the patient—the metabolic and nutritional needs of the human body (easy for students to understand)—as opposed to explanations of complex chemical theory. Mark's Basic empahsizes clinical correlations throughout the text and links biochemical concepts to physiology and pathophysiology, using patient vignettes as the context. These specific and memorable mock patient cases are followed throughout the chapter to pose questions, illustrate core concepts, and help students remember and apply biochemical priniciples within the context of clinical practice.
"Basic Concepts in Biochemistry has just one goal: to review the toughest concepts in biochemistry in an accessible format so your understanding is through and complete."--BOOK JACKET.
A knowledge of the practical aspects of clinical biochemistry is essential for medical students to understand the diagnostic and prognostic status of any ailment. This book addresses and compiles some of the invariably used protocols in biochemistry with the aim to enhance the understanding of the basics behind following the methodologies, including those for urine analysis; carbohydrate, protein, and lipid analyses; and diabetes and lipid profile. It discusses organ function tests, such as liver, renal, or pancreatic function tests, in addition to exploring pH and buffer, colorimeter, spectrophotometer, chromatography, electrophoresis, enzyme-linked adsorbent assay, radioimmunoassay, biosensor/glucometer, fructosamine, DNA isolation, mass spectrometry, flow cytometry, centrifugation, automation, cerebrospinal fluid examination, and oxalic acid test. The book also touches upon the basics of laboratory safety rules and laboratory glassware. The book is helpful for students to understand the relationship between quality management and specimen collection and processing them for their biological functions. It is well-suited for academic courses, including undergraduate, graduate, postgraduate, and professional courses and covers the practical syllabus for MBBS, BDS, BSc MLT, BSc nursing, and MSc students. For undergraduate students in the disciplines of biochemistry, molecular biology, chemistry, and other related biological sciences, the book forms an integral part of the syllabus with its case studies, MCQs, biochemistry, OSPEs, etc. It also demonstrates the actual experiments for understanding the concepts better and is a must read for students to acquire skills to work with a clinical biochemistry laboratory.
Unit 1: Introduction of Clinical Biochemistry 1. Laboratory Apparatus and Equipment, Good and Safe Laboratory Practice, and Waste Disposal Systems in Laboratory Unit 2: Qualitative Experiments and their Clinical Applications 1. Analysis of Carbohydrates 2. Analysis of Proteins 3. Analysis of Physical and Chemical Composition of Physiological Urine 4. Identify, Perform and Interpret Pathological Urine Analysis and Correlate it with Pathological States Unit 3: Quantitative Experiments and their Clinical Interpretation 1. Principle of Colorimetry 2. Principle of Spectrophotometry 3. Estimation of Blood Glucose 4. Glucose Tolerance Test and Glycated Hemoglobin 5. Liver Function Test 6. Kidney Function Test 7. Lipid Profile (Atherogenic Profile) 8. Estimation of Serum Calcium and Serum Phosphorus Unit 4: Self-Directed Learning Exercises 1. pH Meter 2. Water Homeostasis and Estimation of Na+ and K+ with ISE Analyzer 3. Arterial Blood Gas Analyzer 4. Chromatography 5. Electrophoresis 6. Enzyme-linked Immunosorbent Assay 7. Antigen-Antibody Interaction (Immunodiffusion) 8. Quality Control in Clinical Laboratory 9. DNA Isolation from Blood and Tissue Unit 5: Early Clinical Exposure Exercises and Reflective Writing 1. Analysis of Cerebrospinal Fluid 2. Thyroid Function Test 3. Pancreatic Function Tests 4. Disorders of Acid-Base Balance Unit 6: Attitude, Ethics and Communication (AETCOM) Modules 1. Introduction of Clinical Methods 2. What does it Mean to be a Doctor? 3. What does it Mean to be a Patient? 4. The Doctor-Patient Relationship 5 The Foundations of Communications Unit 7: Biochemical Calculations and Reference Range 1. Preparations of Buffers and Solutions 2. Reference Value of Various Biochemical Parameters Integration with Medicine Unit 8: Practical Spots in Biochemistry 1. Practical Spots in Biochemistry Unit 9: Competency-Based Assessment for Practical Biochemistry 1. Competency-based Assessment for Practical Biochemistry Index
We are very pleased to put forth the revised edition of 'Laboratory Manual of Biochemistry and Clinical Pathology'. We have incorporated all the suggestions, modified it to make it easier, student friendly and relevant in terms of achieving curriculum outcome. We are very much thankful to all the learned teachers who have given their feedback whole-heartedly. We have even incorporated the changes in this manual based on the feedback given by the teachers from all the institutes. Now, we believe that the manual has been fulfilling the aspirations of biochemistry teachers and students too. This manual is prepared as per PCI Education Regulations, 2020 for Diploma Course in Pharmacy. The methods of all the experiments are reviewed and added from the recent research papers, so that the advancement in the methods or apparatus can be addressed. This manual is designed for 'outcome-based education' and each experiment is arranged in a uniform way such as practical significance, practical outcomes (PrOs) and its mapping with course outcomes, minimum theoretical background, resources used, procedure, precautions, observations, result, conclusion, references and related questions. Moreover, assessment scheme is also given to help the student and teacher to know what to be assessed. During the laboratory period, you will have to multitask, while you are doing the experiment. It is essential to document properly what you do and what you observe while doing the practical. Always plan your work ahead and think about what you are doing, why you are doing it, what is happening, and what you can conclude from your experiment.
Now over 70,000 copies sold! This comprehensively revised edition of Clinical Biochemistry offers essential reading for today’s students of medicine and other health science disciplines – indeed, anyone who requires a concise, practical introduction to the subject. Topics are clearly presented in a series of double-page ‘learning units’, each covering a particular aspect of clinical biochemistry. Four sections provide a core grounding in the subject: Introducing clinical biochemistry gives an insight into how modern hospital laboratories work, and includes an entirely new series of learning units on the interpretation of test results Core biochemistry covers the bulk of routine analyses, and their relevance to the clinical setting Endocrinology provides an overview of endocrine investigations as well as a practical approach to thyroid, adrenal, pituitary and gonadal function testing Specialised investigations embraces an assortment of other topics that students may encounter This edition represents the most radical revision of the book to date. Every learning unit has been examined and updated to reflect current developments and clinical best practice. Entirely new material includes a series of learning units on interpretation and analytical aspects of clinical biochemistry. Coverage of fluid biochemistry is now more comprehensive. New "Want to know more?" links throughout the book point readers to relevant further information. (Printed version) now includes the complete eBook version for the first time – downloadable for anytime access and enhanced with new, interactive multiple choice questions for each section, to test your understanding and aid exam preparation
This textbook explains the basic principles of Biochemistry, Nutrition and Dietetics and their application to health and disease. It presents core information to introduce basic concepts and thereby apply the acquired knowledge in nursing practice. Third edition is comprehensively updated to meet the constantly changing health needs of people. Content has been reorganized and significant changes have been made during the development of the text to include addition of a new section on biochemistry and recent updates in the Nutrition section as per the revised syllabus outlined by the Indian Nursing Council. This book can be used by students and teachers of Biochemistry, Nutrition, Dietetics, Nursing, Medicine, and other health sciences. Highlights: Now in FULL COLOR! UPDATED! As per the revised Indian Nursing Council syllabus NEW! Section on biochemistry comprising 8 chapters “Nutrition” included in chapter Therapeutic Diets to address the basic nutrition needs of affected patients NEW! Chapter Nutrition Deficiency Disorders included which covers causes, signs and symptoms, and management of important and prevalent disease conditions such as severe acute malnutrition, childhood obesity, and deficiency disorders of vitamins and minerals UPDATED! Recommended dietary allowances, IYCF guidelines, anemia in pregnancy and adolescence, and nutrition education Recipes for different types of diet and sample menus for important diseases included for ready reference Important topics like “Calculation of nutritive value of foods” included with examples for easy understanding Enzymes of diagnostic importance for various diseases discussed Metabolism of carbohydrates, proteins, and lipids illustrated for better understanding Content presented in a student friendly manner complemented with plenty of illustrations, flowcharts, and tables Chapter-end summaries for quick review and Self-Assessment section as per University examination pattern An extensive glossary included.
Protocols in Biochemistry and Clinical Biochemistry offers clear, applied instruction to fundamental biochemistry methods and protocols, from buffer preparation to nucleic acid purification, protein, lipid, carbohydrate, and enzyme testing, and clinical testing of vitamins, glucose and cholesterol levels, among other diagnostics. Each protocol is illustrated with step-by-step instructions, labeled diagrams, and color images, as well as a thorough overview of materials and equipment, precursor techniques, safety considerations and standards, analysis and statistics, alternative methods and troubleshooting. Includes full listings and discussion of materials and equipment, precursor techniques, safety considerations and standards, analysis and statistics, alternative methods and troubleshooting Features clear, step-by-step protocols and instructions with color diagrams and images