[PDF] Avidin Biotin Interactions eBook

Author : Robert J. McMahon
Publisher : Springer Science & Business Media
Page : 239 pages
File Size : 12,66 MB
Release : 2008-02-26
Category : Science
ISBN : 1588295834

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This book is specifically about the application of the extremely powerful interaction between the protein avidin or its homologues and the vitamin biotin and some of its homologues. With excellent descriptions of laboratory protocols written by expert researchers, this volume is equally perfect for the student or the professional laboratory scientist.

Author : S. Avrameas
Publisher : Elsevier Science & Technology
Page : 428 pages
File Size : 32,1 MB
Release : 1983
Category : Medical
ISBN :

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Aandacht voor de enzymatische-immunocytochemie met de nadruk op intracellulaire penetratie van gelabelde antilichamen en de toepassing van "double staining"-methodes of monoclonale antilichamen in normale en in diverse experimentele of pathologische omstandigheden; kwantitatieve aspecten van immuno-enzymatische technieken, zoals nieuwe technische vindingen en toepassingen op verschillende gebieden, bijvoorbeeld biologie, humane en veterinaire geneeskunde, fytopathologie en experimentele ziekten

Author : Meir Wilchek
Publisher :
Page : 794 pages
File Size : 50,29 MB
Release : 1990
Category : Avidin
ISBN :

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The avidin-biotin complex has been used for isolation (affinity chromatography), localization (affinity cytochemistry, cell cytometry, and blotting technology), and diagnostics (immunoassay, histopathology, and gene probes). Recently, usage of the system has been extended to include other areas. This volume covers these new applications and methodologies including hybridoma technology, bioaffinity sensors, affinity targeting, and drug delivery, as well as cross-linking, immobilization, and fusogenic studies.

Author : Greg T. Hermanson
Publisher : Academic Press
Page : 1233 pages
File Size : 29,88 MB
Release : 2010-07-26
Category : Science
ISBN : 0080568726

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Bioconjugate Techniques, 2nd Edition, is the essential guide to the modification and cross linking of biomolecules for use in research, diagnostics, and therapeutics. It provides highly detailed information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules. It also describes dozens of reactions with details on hundreds of commercially available reagents and the use of these reagents for modifying or cross linking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers. A one-stop source for proven methods and protocols for synthesizing bioconjugates in the lab Step-by-step presentation makes the book an ideal source for researchers who are less familiar with the synthesis of bioconjugates More than 600 figures that visually describe the complex reactions associated with the synthesis of bioconjugates Includes entirely new chapters on the latest areas in the field of bioconjugation as follows: Microparticles and nanoparticlesSilane coupling agentsDendrimers and dendronsChemoselective ligationQuantum dotsLanthanide chelatesCyanine dyesDiscrete PEG compoundsBuckyballs,fullerenes, and carbon nanotubesMass tags and isotope tagsBioconjugation in the study of protein interactions

Author : Robert J. McMahon
Publisher : Humana Press
Page : 232 pages
File Size : 40,20 MB
Release : 2008-02-26
Category : Science
ISBN : 9781588295835

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This book is specifically about the application of the extremely powerful interaction between the protein avidin or its homologues and the vitamin biotin and some of its homologues. With excellent descriptions of laboratory protocols written by expert researchers, this volume is equally perfect for the student or the professional laboratory scientist.

Author : U.B. Sleytr
Publisher : Springer Science & Business Media
Page : 217 pages
File Size : 42,91 MB
Release : 2013-06-29
Category : Science
ISBN : 1447134796

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Immobilized functional biomolecules, particularly enzymes, are important tools in biotechnology, biochemistry, biochemical engineering, biomedicine and biosensor research. This book provides an introduction and overview of selected major areas of the science and technology of immobilized systems. The chapters are intended as an introduction and overview to these interdisciplinary areas, as well as a source of practical details and of new research trends. This book will be useful for scientists, technologists, academics and students in direct and related fields.

Author :
Publisher : Academic Press
Page : 310 pages
File Size : 27,1 MB
Release : 2020-02-10
Category : Science
ISBN : 0128191295

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Chemical and Synthetic Biology Approaches to Understand Cellular Functions - Part C, Volume 633, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial. This release includes sections on Next generation probes for molecular imaging in cells, Competitive binding assay for biotin and biotin derivatives, based on avidin and biotin-4-fluorescein, Converting avidin to bind ligands other than biotin, especially steroids, Chemoenzymatic Labeling Strategy, Engineered Siderophores, Small molecules to inhibit bacterial population behavior, NMR tube bioreactor, Small molecule controlled RAS activation system, Small molecule regulated Cas9, the Design and application of synthetic receptors, and much more. Contains the authority of authors who are leaders in their field Provides a comprehensive source on new methods and research in enzymology

Author : Shiyu Wang
Publisher : Springer Nature
Page : 123 pages
File Size : 37,1 MB
Release : 2021-03-31
Category : Technology & Engineering
ISBN : 9811612129

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In this monograph, the graphene-based field-effect transistor (FET) biosensors are shown to be an emerging sensing platform. Divided into two parts the first set of chapters are devoted to basic knowledge of graphene, graphene FET and its biosensing. In the second part of this book the applications of graphene FET biosensors combined with various biotechnologies are presented. As well as discussing the existing technologies the authors also introduce their own ideas and concepts. Finally the remaining problems in graphene FET biosensors are discussed, along with proposed solutions and prospects for future applications. This monograph allows readers to grasp the basic knowledge and future direction of graphene-based FET biosensors.

Author : Rawil Fakhrullin
Publisher : Royal Society of Chemistry
Page : 266 pages
File Size : 34,14 MB
Release : 2014-07-24
Category : Medical
ISBN : 1849739021

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A summary of the recent achievements in surface-functionalised cells including fabrication, characterisation, applications and nanotoxicity.

Author : Victor C. Yang
Publisher : Springer Science & Business Media
Page : 370 pages
File Size : 44,92 MB
Release : 2012-12-06
Category : Science
ISBN : 1461541816

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A biosensor is a device in which a bioactive layer lies in direct contact with a transducer whose responses to change in the bioactive layer generate eloctronic signals for interpretation. The bioactive layer may consist of membrane-bound enzymes, anti-bodies, or receptors. The potential of this blend of electronics and biotechnology includes the direct assay of clinically important substrates (e.g. blood glucose) and of substances too unstable for storage or whose concentrations fluctuate rapidly. Written by the leading researchers in the field, this book reflects the most current developments in successfully constructing a biosensor. Major applications are in the fields of pharmacology, molecular biology, virology and electronics.