[PDF] Ultrastructure Of The Connective Tissue Matrix eBook

Author : P. Motta
Publisher : Springer Science & Business Media
Page : 224 pages
File Size : 45,39 MB
Release : 2012-12-06
Category : Medical
ISBN : 1461328314

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In recent years, the techniques of electron microscopy have developed so widely and rapidly that they now cover the fields of research once the unique ll:panage of sister research techniques such as biochemistry, physiology, immunology, X-ray diffraction, etc. It is now possible to reach molecular and submolecular levels, making this technique indispensable in every type of research. Electron microscopy alone often provides enough information to solve given problems. In the field of the connective tissue matrix, knowledge of the molecular structure of collagen, pro teoglycans and elastin and their interaction has been to a large extent elucidated by electron microscopy. The field over which electron microscopy ranges in the investigation of the connective tissue matrix is so wide that the aim of this volume is to collect the main ultrastructural acquisitions disseminated in various journals and monographs in one book. The intent ofthis volume is to: (a) integrate different and new microscopic methods and review the results of such an integrative approach; (b) present a comprehensive ultrastructural account of selected aspects of the field; (c) point out gaps or controversial topics in our knowledge; (d) outline pertinent future research and expansion of the subject.

Author : Laszlo Modis
Publisher : CRC Press
Page : 295 pages
File Size : 18,40 MB
Release : 2018-05-04
Category : Science
ISBN : 1351083732

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This book describes analysis techniques and results of topics such as physical backgrounds, chemical backgrounds, and principal methods of topo-optical reactions used in ultrastructure research of the ECM; orientation patterns of GAGs and collagen in different tissues/cartilage, cornea, kidney basement membranes, and skin; factors involved in the formation of submicroscopically ordered matrix structure; and cell-matrix interactions, including cytoskeleton-cell-membrane-matrix relationships. A summarization of the advantages and limitations of polarization microscopy compared to electron microscopy in ultracellular research is also included. Cell biologists, histologists, pathologists, and biochemists in connective tissue research will find this book to be an invaluable reference tool.

Author : E. Bonucci
Publisher : Springer Science & Business Media
Page : 302 pages
File Size : 18,79 MB
Release : 2012-12-06
Category : Medical
ISBN : 1461314879

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The calcified tissues have fundamental functions in the biology of organisms, not only because their strength, solidity, and elasticity permit movement and mechanical activities, and protect soft tissues against traumatic forces, but also on account of their role in mineral homeostasis. For this reason, extensive investigation in the last 30 years has provided much to explain the complex chemical and physical processes occurring in cells and matrices composing the skeleton, and their alterations in pathological conditions. The use of ultrastructural methods such as immunocytochemistry, scanning and transmission electron microscopy, cytoautoradiography, freeze/fracture etching, high voltage, etc. has proven to be of great value when applied to cells and matrix components of bone and cartilage, in spite of the technical difficulties due to the hardness of these tissues. However, available information on this subject is disseminated in a variety of scientific and medical articles. This volume is an attempt to collect together the most significant data on the ultrastructure of cartilage and bone in normalcy and pathology. Obviously, it cannot be a complete report of all these data, its principal aim being that of: a) giving a comprehensive statement of the results concerning the basic structures common to these tissues, especially collagen fibrils, noncollagenous proteins, and proteoglycans, and their relationships with the mineral substance (for which another volume of this series can also be consulted; see Ruggeri A. , Motta P. M. (eds.

Author : David W. L. Hukins
Publisher :
Page : 328 pages
File Size : 20,71 MB
Release : 1990
Category : Collagen
ISBN :

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This book is a sequel to Volume 5 of the series, Connective Tissue Matrix. The emphasis in the present volume is on changes which occur in connective tissue matrix and how its components influence biological properties. Changes include alteration of structure and loss of fluid, in response to mechanical loading, and biological changes occurring during development, degeneration and ageing. The chapters illustrate the wide range of disciplines involved in current research but are not intended to provide a comprehensive review of the subject. Volume 11 of the series, Calcified Tissue, deals with related tissues whose composition and properties are modified by calcification.

Author : Robert Mecham
Publisher : Springer Science & Business Media
Page : 429 pages
File Size : 18,41 MB
Release : 2011-02-16
Category : Science
ISBN : 3642165559

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Knowledge of the extracellular matrix (ECM) is essential to understand cellular differentiation, tissue development, and tissue remodeling. This volume of the series “Biology of Extracellular Matrix” provides a timely overview of the structure, regulation, and function of the major macromolecules that make up the extracellular matrix. It covers topics such as collagen types and assembly of collagen-containing suprastructures, basement membrane, fibronectin and other cell-adhesive glycoproteins, proteoglycans, microfibrils, elastin, fibulins and matricellular proteins, such as thrombospondin. It also explores the concept that ECM components together with their cell surface receptors can be viewed as intricate nano-devices that allow cells to physically organize their 3-D-environment. Further, the role of the ECM in human disease and pathogenesis is discussed as well as the use of model organisms in elucidating ECM function.

Author : Bela Suki
Publisher : Academic Press
Page : 284 pages
File Size : 14,82 MB
Release : 2021-11-27
Category : Technology & Engineering
ISBN : 0128226056

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Structure and Function of the Extracellular Matrix: A Multiscale Quantitative Approach introduces biomechanics and biophysics with applications to understand the biological function of the extracellular matrix in health and disease. A general multiscale approach is followed by investigating behavior from the scale of single molecules, through fibrils and fibers, to tissues of various organ systems. Through mathematical models and structural information, quantitative description of the extracellular matrix function is derived with tissue specific details. The book introduces the properties and organization of extracellular matrix components and quantitative models of the matrix, and guides the reader through predicting functional properties. This book integrates evolutionary biology with multiscale structure to quantitatively understand the function of the extracellular matrix. This approach allows a fresh look into normal functioning as well as the pathological alterations of the extracellular matrix. Professor Suki’s book is written to be useful to undergraduates, graduate students, and researchers interested in the quantitative aspects of the extracellular matrix. Researchers working in mechanotransduction, respiratory and cardiovascular mechanics, and multiscale biomechanics of tendon, cartilage, skin, and bone may also be interested in this book. Examines the evolutionary origins and consequences of the extracellular matrix Delivers the first book to quantitatively treat the extracellular matrix as a multiscale system Presents problems and a set of computational laboratory projects in various chapters to aid teaching and learning Provides an introduction to the properties and organization of the extracellular matrix components

Author :
Publisher : Academic Press
Page : 470 pages
File Size : 12,81 MB
Release : 2018-01-05
Category : Science
ISBN : 0128122986

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Methods in Extra Cellular Matrix, Volume 142, a new volume in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Unique to this updated volume are sections devoted to Elastin, Quantification of collagen and elastin, Fibrillins, Lysyl oxidase, Fibulins, Matrilins, Hyaluronic Acid, Small leucine-rich proteoglycans, Syndecans, Fibronectin, SPARC, Thrombospondins, Tenascins, Collagen IV, Multi-photon analysis of ECM, Cell-derived extracellular matrices, Laminins, Fibrillar Collagens, Imaging ECM in developing embryos, Analysis of Matrix Degradation, Ultrastructural analysis of ECM, Versican and Large proteoglycans, and an ECM crosslink analysis. This series covers a wide array of topics about the extracellular matrix, including an understanding of crucial proteins and glycoproteins components of ECM. Contains contributions from experts in the field from across the world Covers a wide array of topics on the extracellular matrix, including an understanding crucial proteins and the glycoproteins components of ECM Includes analysis based topics, such as quantification of collagen and elastin, mulit-photon analysis of ECM and ECM crosslink analysis

Author : Margit Pavelka
Publisher : Springer Science & Business Media
Page : 340 pages
File Size : 46,90 MB
Release : 2005-12-05
Category : Medical
ISBN : 3211263926

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The period between 1950 and 1980 were the golden It also provides unique insights into how pathological years of transmission electron microscopy and pro- processes affect cell organization. duced a plethora of new information on the structure of This information is vital to current work in which cells that was coupled to and followed by biochemical the emphasis is on integrating approaches from p- and functional studies. TEM was king and each micro- teomics, molecular biology, molecular imaging and graph of a new object produced new information that physiology, and pathology to understand cell functions led to new insights on cell and tissue organization and and derangements in disease. In this current era, there is their functions. The quality of data represented by the a growing tendency to substitute modern light mic- images of cells and tissues had been perfected to a very scopic techniques for electron microscopy because it is high level by the great microscopists of the era including less technically demanding and is more readily available Palade, Porter, Fawcett, Sjostrand, Rhodin and many to researchers. This atlas reminds us that the infor- others. At present, the images that we see in leading tion obtained by electron microscopy is invaluable and journals for the most part do not reach the same techni- has no substitute.

Author : M.B. Mathews
Publisher : Springer Science & Business Media
Page : 332 pages
File Size : 23,99 MB
Release : 2012-12-06
Category : Science
ISBN : 3642809049

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This book deals primarily with the principal extracellular macromole cules of animal connective tissues. It attempts to answer some general questions about the biological organization of the tissues: What is the nature of this organization at various dimensional levels? What functions does the organization serve? How has it evolved? I have given major emphasis to the structures and properties of the macromolecular components of extracellular matrices from a wide range of invertebrates and vertebrates. In doing so, however, I have treated cursorily many important aspects of connective tissue biology that appeared to be only indirectly relevant to the principal questions asked. On the other hand, I have not hesitated to search for broad biological principles outside the prevailing conceptual boundaries of animal connective tissues and the lower molecular dimensional levels. The numerous speculations presented will, I hope, stimulate the reader to further thought and investigation. Acknowledgements My previously unpublished data that have been included in this volume could not have been obtained without the generous gifts of specimens and other assistance by a number of individuals and institutions. These are Dr. G. BERENSON of Louisiana State University, Drs. W. P. BRAKER and D. ZUMWALD of the Shedd Aquarium, Dr. S. H. CHUANG of the Univer sity of Singapore, Dr. L. G. CLARK and Mr. U. M. VARELA-DIAZ of the University of Pennsylvania, the Government of Nicaragua, Dr. E. CLARK of Cape Haze Marine Laboratories, Mr. C. E.

Author : Jouni Uitto
Publisher : CRC Press
Page : 576 pages
File Size : 34,91 MB
Release : 2021-07-29
Category : Science
ISBN : 1000447227

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This book is a collection of works that canvass many of the recent developments in various areas of connective tissue research. It focuses on the structure of the components, molecular organization and pathology of the extracellular matrix.