[PDF] Methods Of Protein Separation eBook

Author : Nicholas Catsimpoolas
Publisher : Springer
Page : 290 pages
File Size : 12,62 MB
Release : 2013-04-18
Category : Science
ISBN : 147571257X

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This open-end treatise on methods concerning protein separation had its beginning in an American Chemical Society symposium entitled "Con temporary Protein Separation Methods" which was held in Atlantic City, New Jersey in September 1974. The purpose of the symposium-and subse quently of the present work-was to review the available modern techniques and underlying principles for achieving one of the very important tasks of experimental biology, namely the separation and characterization of proteins present in complex biological mixtures. Physicochemical characterization was covered only as related to the parent method of fractionation and there fore involved mostly mass transport processes. Additionally, the presentation of methods for gaini. ng insight into complex interacting protein profiles was considered of paramount importance in the interpretation of separation patterns. Finally, specific categories of proteins (e. g. , chemically modified, deriving from a specific tissue, conjugated to different moieties, etc. ) require meticulous trial and selection andjor modification of existing methodology to carry out the desired separation. In such cases, the gained experience provides valuable guidelines for further experimentation. Although powerful techniques exist today for the separation and related physicochemical characterization of proteins, many biological fractionation problems require further innovations. It is hoped that the description in the present treatise of some of the available separation tools and their limitations will provide the necessary integrated background for new developments in this area.

Author : Jan-Christer Janson
Publisher : John Wiley & Sons
Page : 712 pages
File Size : 23,67 MB
Release : 1998
Category : Medical
ISBN : 9780471186267

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This is a state-of-the-art sourcebook on modern high-resolution biochemical separation techniques for proteins. It contains all the basic theory and principles used in protein chromatography and electrophoresis.

Author : Robert K. Scopes
Publisher : Springer Science & Business Media
Page : 342 pages
File Size : 14,73 MB
Release : 2013-04-17
Category : Science
ISBN : 1475719574

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New textbooks at alllevels of chemistry appear with great regularity. Some fields like basic biochemistry, organic reaction mechanisms, and chemical thermodynamics are weil represented by many excellent texts, and new or revised editions are published sufficiently often to keep up with progress in research. However, some areas of chemistry, especially many of those taught at the graduate level, suffer from a reallack ofup-to-date textbooks. The most serious needs occur in fields that are rapidly changing. Textbooks in these subjects usually have to be written by scientists actually involved in the research which is advancing the field. lt is not often easy to persuade such individuals to settime aside to help spread the knowledge they have accumu lated. Our goal, in this series, is to pinpoint areas of chemistry where recent progress has outpaced what is covered in any available textbooks, and then seek out and persuade experts in these fields to produce relatively concise but instructive introductions to their fields. These should serve the needs of one semester or one quarter graduate courses in chemistry and biochemistry. In some cases the availability of texts in active research areas should help stimulate the creation of new courses. NewYork CHARLES R. CANTOR Preface to the Second Edition The original plan for the first edition of this book was to title it Enzyme Purification: Princip/es and Practice.

Author : Richard R Burgess
Publisher : Academic Press
Page : 915 pages
File Size : 21,57 MB
Release : 2009-11-03
Category : Science
ISBN : 0080923178

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Guide to Protein Purification, Second Edition provides a complete update to existing methods in the field, reflecting the enormous advances made in the last two decades. In particular, proteomics, mass spectrometry, and DNA technology have revolutionized the field since the first edition’s publication but through all of the advancements, the purification of proteins is still an indispensable first step in understanding their function. This volume examines the most reliable, robust methods for researchers in biochemistry, molecular and cell biology, genetics, pharmacology and biotechnology and sets a standard for best practices in the field. It relates how these traditional and new cutting-edge methods connect to the explosive advancements in the field. This "Guide to" gives imminently practical advice to avoid costly mistakes in choosing a method and brings in perspective from the premier researchers while presents a comprehensive overview of the field today. Gathers top global authors from industry, medicine, and research fields across a wide variety of disciplines, including biochemistry, genetics, oncology, pharmacology, dermatology and immunology Assembles chapters on both common and less common relevant techniques Provides robust methods as well as an analysis of the advancements in the field that, for an individual investigator, can be a demanding and time-consuming process

Author : E. L. V. Harris
Publisher : Oxford University Press, USA
Page : 344 pages
File Size : 32,5 MB
Release : 1989
Category : Language Arts & Disciplines
ISBN :

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This book brings together useful practical protocols for the purification of proteins, concentrating on the uses of buffers and different means of separation, by charge, activity and size. Diverse applications of these methods can be found in the companion volume.

Author : Arne Staby
Publisher : John Wiley & Sons
Page : 608 pages
File Size : 39,67 MB
Release : 2017-02-02
Category : Technology & Engineering
ISBN : 1119031176

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Preparative Chromatography for Separation of Proteins addresses a wide range of modeling, techniques, strategies, and case studies of industrial separation of proteins and peptides. • Covers broad aspects of preparative chromatography with a unique combination of academic and industrial perspectives • Presents Combines modeling with compliantce useing of Quality-by-Design (QbD) approaches including modeling • Features a variety of chromatographic case studies not readily accessible to the general public • Represents an essential reference resource for academic, industrial, and pharmaceutical researchers

Author : Shawn Doonan
Publisher : Springer Science & Business Media
Page : 406 pages
File Size : 40,31 MB
Release : 2008-02-02
Category : Science
ISBN : 1592595456

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Hans Neurath has written that this is the second golden era of enzymology {Protein Science [1994], vol. 3, pp. 1734—1739); he could with justice have been more general and referred to the second golden age of protein chemistry. The last two decades have seen enormous advances in our understanding of the structures and functions of pro teins arising on the one hand from improvements and developments in analytical techniques {see the companion volume, Basic Protein and Peptide Protocols, in this series) and on the other hand from the tech nologies of molecular genetics. Far from turning the focus away from protein science, the ability to isolate, analyze, and express genes has increased interest in proteins as gene products. Hence, many laborato ries are now getting involved in protein isolation for the first time, either as an essential adjunct to their work in molecular genetics or because of a curiosity to know more about the products of the genes that they have been studying. Protein Purification Protocols is aimed mainly at these newcom ers to protein purification, but it is hoped that it will also be of value to established practitioners who may find here techniques that they have not tried, but which might well be most applicable in their work. With the exception mainly of the first and last chapters, the format of the contributions to the present book conform to the established format of the Methods in Molecular Biology series.

Author : Gary B. Smejkal
Publisher : CRC Press
Page : 532 pages
File Size : 38,38 MB
Release : 2005-12-12
Category : Medical
ISBN : 1420028057

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Driven by the widespread growth of proteomic practices, protein separation techniques have been refined to minimize variability, optimize particular applications, and adapt to user preferences in the analysis of proteins. Separation Methods in Proteomics provides a comprehensive examination of all major separation techniques for proteomic

Author : David W. Burden
Publisher : Springer Science & Business Media
Page : 338 pages
File Size : 29,74 MB
Release : 1995-11-01
Category : Science
ISBN : 9780817638436

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A textbook for an undergraduate or professional training course for students with a solid background in general biology and chemistry, and hopefully some organic chemistry; does not assume any microbiology and biochemistry. Explains the laboratory techniques and skills for characterizing and purifying a protein and for cloning the associated gene, and the strategies and rationale for the research process itself. The examples use readily available materials and organisms to keep the cost down. Annotation copyright by Book News, Inc., Portland, OR