[PDF] Colloidal Gold Post Embedding Immunocytochemistry eBook

Author :
Publisher : Elsevier
Page : 517 pages
File Size : 26,74 MB
Release : 2012-12-02
Category : Technology & Engineering
ISBN : 0323139027

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Since its introduction in 1971, the development and application of colloidal gold as a marker in electron microscopy has been phenomenal. Colloidal gold has become the method of choice in immunocytochemistry and many areas of cell biology. This universal method is applicable to most microscopical systems including optical microscopy; scanning, transmission, and high voltage electron microscopy; photoelectron, photon, fluorescent darkfield, and epipolarization microscopy. Colloidal gold allows high and low resolution studies, enzyme and nucleic acid labeling, study of dynamic cellular processes, and virus detection. This book is among the first available to cover the principles and methodology of colloidal gold in microscopy. Methods are described step by step, to enable researchers to learn these complex procedures solely by reference to these books Problems and limitations of techniques are discussed Guides users to avoid problems and choose the correct procedures for specific applications Contributors are eminent authorities in their fields

Author :
Publisher : Elsevier
Page : 565 pages
File Size : 35,49 MB
Release : 2012-12-02
Category : Nature
ISBN : 0323138810

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Since its introduction in 1971, the development and application of colloidal gold as a marker in electron microscopy has been phenomenal. This state-of-the art, multi-volume treatise provides researchers, technicians, teachers, and students with the most comprehensive coverage of the principles and methodology of colloidal gold microscopy available today. This universal method is applicable to most microscopical systems including optical microscopy, scanning, transmission and high voltage electron microscopy, and photoelectron, photon, fluorescent darkfield and epipolarization microscopy. Colloidal gold allows high and low resolution studies, enzyme and nucleic acid labeling, study of dynamic cellular processes, and virus detection. Principles, methods, and applications of colloidal gold methodology in cytochemistry and immunochemistry Methods for preparing colloidal gold particles of different sizes Protein A-gold, protein G-gold, and lectin-gold techniques The use of resins and thin cryosections Multiple labeling

Author : M. A. Hayat
Publisher : CRC Press
Page : 344 pages
File Size : 23,28 MB
Release : 1995-08-03
Category : Science
ISBN : 9780849324499

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This book discusses the principles, methods, and applications of immunogold-silver staining (IGSS) to biomedical areas. It focuses on the latest advances in the dynamic and progressive field of IGSS.

Author : Gerhard W. Hacker
Publisher : CRC Press
Page : 265 pages
File Size : 39,85 MB
Release : 2002-04-17
Category : Medical
ISBN : 1420040235

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Immunogold silver staining is one of the most sensitive techniques available for visualizing the location of antibodies and nucleotide probes that have been bounded to specific antigens or to nucleotide sequences. As gold and silver staining continues to advance research in molecular morphology, this book presents the information you need to know a

Author : Julian E. Beesley
Publisher : Oxford University Press, USA
Page : 72 pages
File Size : 14,38 MB
Release : 1989
Category : Language Arts & Disciplines
ISBN :

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The colloidal gold probe is the most recent immunocytochemical marking technique to have been developed for the detection of antigens in situ. The major advantages of colloidal gold probes are that they are small, dense probes of wide applicability in the biological sciences. The probe can be used for the localization of specific proteins in conjunction with a wide range of microscopical and non-microscopical techniques. This book aims to describe the established techniques of gold probe production and immunolabelling. Each technique is summarized in a table which can be used in the laboratory without recourse to the text. Original references are also given to enable readers to trace the origins of each technique. The book can therefore be used both as a bench-top manual and as a guide to further reading.

Author : Alexander D. Hyatt
Publisher : CRC Press
Page : 476 pages
File Size : 17,48 MB
Release : 1992-11-10
Category : Science
ISBN : 9780849367595

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This book presents a wide variety of immuno-gold techniques for use in virus diagnosis and research. Protocols are presented for state-of-the-art techniques, including in situ hybridization, freeze substitution, and the utilization of ultra-small probes and replicas for use by virologists and electron microscopists identifying and studying viruses, their components, and replication in cells. The procedures are described by eminent scientists and are pertinent to both experienced researchers and newcomers to this field who are interested in the localization of low antigenic mass structures.

Author : Lorette C. Javois
Publisher : Springer Science & Business Media
Page : 928 pages
File Size : 11,91 MB
Release : 2008-02-02
Category : Science
ISBN : 1592592139

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Lorette Javois' timely new 2nd edition revises and updates her widely acclaimed collection of step-by-step immunocytochemical methods, one that is now used in many biological and biomedical research programs. The methods are designed for researchers and clinicians who wish to visualize molecules in plant or animal embryos, tissue sections, cells, or organelles. In addition to cutting-edge protocols for purifying and preparing antibodies, light microscopic analysis, confocal microscopy, FACS, and electron microscopy, this revised edition contains many new methods for applying immunocytochemical techniques in the clinical laboratory and in combination with in situ hybridization.