[PDF] Assays For Bioactivity eBook

Author : Kurt Hostettmann
Publisher :
Page : 384 pages
File Size : 34,53 MB
Release : 1991
Category : Science
ISBN :

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This new series, Methods in Plant Biochemistry, is an authoritative reference on current techniques in the various fields of plant biochemical research. Each volume in the series, under the expert guidance of a guest editor, addresses a particular group of plant compounds.**The most current and useful methods of analysis are described, with detailed discussions of the development, protocols, and suitability of each technique. Case treatments, diagrams, chemical structures, reference data, and properties are featured where appropriate, along with a full list of references to the specialist literature.**Conceived as a practical companion to the Biochemistry of Plants, edited by P.K. Stumpf and E.E. Conn, no plant biochemical laboratory can afford to be without this comprehensive and up-to-date reference. Addresses the laboratory analysis of all major plant compounds**Illustrates authoritative and detailed practical instructions and recipes for analytical methods**Describes assays suitable for showing biological or pharmacological properties in crude plant extracts

Author : K. (Ed.) HOSTETTMANN
Publisher :
Page : 360 pages
File Size : 33,83 MB
Release : 1991
Category :
ISBN :

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Crown gall tumours on potato discs and brine shrimp lethality: two simple bioassays for higher plant screening and fractionation. Assays for antifungal activity. Screening methods for antibacterial and antiviral agents from higher plants. Assays related to cancer drug discovery. Assays for antimalarial and amoebicidal activities. Assays for molluscicidal, cercaricidal and piscicidal activities. Screening techniques for plant-insect interactions. Assay methods for antihepatotoxic activity. Assays for pharmacological activity. A primer in plant electrophysiological methods. Organ culture and manipulation.

Author : Fidel Toldra
Publisher : Academic Press
Page : 819 pages
File Size : 40,86 MB
Release : 2021-06-17
Category : Science
ISBN : 0128214384

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Biologically Active Peptides: From Basic Science to Applications for Human Health stands as a comprehensive resource on bioactive peptide science and applications. With contributions from more than thirty global experts, topics discussed include bioactive peptide science, structure-activity relationships, best practices for their study and production, and their applications. In the interdisciplinary field of bioactive peptides, this book bridges the gap between basic peptide chemistry and human physiology, while reviewing recent advances in peptide analysis and characterization. Methods and technology-driven chapters offer step-by-step guidance in peptide preparation from different source materials, bioactivity assays, analysis and identification of bioactive peptides, encoding bioactive peptides. Later, applications across disease areas and medical specialties are examined in-depth, including the use of bioactive peptides in treating obesity, diabetes, osteoporosis, mental health disorders, food allergies, and joint health, among other disorders, as well as bioactive peptides for sensory enhancement, sports and clinical nutrition, lowering cholesterol, improving cardiovascular health, and driving advances in biotechnology. Discusses the latest advances in bioactive peptide chemistry, functionality and analysis Offers step-by-step instruction in applying new technologies for peptide extraction, protection, production and encoding, as well as employing bioactive peptide sequencing and bioactivity assays in new research Effectively links basic peptide chemistry, human biology and disease Features chapter contributions from international experts across disciplines and applications

Author : Kurt Hostettmann
Publisher :
Page : 384 pages
File Size : 34,82 MB
Release : 1991
Category : Science
ISBN : 9780124610163

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This new series, Methods in Plant Biochemistry, is an authoritative reference on current techniques in the various fields of plant biochemical research. Each volume in the series, under the expert guidance of a guest editor, addresses a particular group of plant compounds.**The most current and useful methods of analysis are described, with detailed discussions of the development, protocols, and suitability of each technique. Case treatments, diagrams, chemical structures, reference data, and properties are featured where appropriate, along with a full list of references to the specialist literature.**Conceived as a practical companion to the Biochemistry of Plants, edited by P.K. Stumpf and E.E. Conn, no plant biochemical laboratory can afford to be without this comprehensive and up-to-date reference. Addresses the laboratory analysis of all major plant compounds**Illustrates authoritative and detailed practical instructions and recipes for analytical methods**Describes assays suitable for showing biological or pharmacological properties in crude plant extracts

Author : C. I. Bliss
Publisher : Elsevier
Page : 189 pages
File Size : 41,7 MB
Release : 2014-05-12
Category : Medical
ISBN : 1483277011

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The Statistics of Bioassay: With Special Reference to the Vitamins, Volume II focuses on the processes, reactions, principles, and approaches involved in the biological assay of vitamins. The publication first offers information on the general principles of biological assay, dosage-response curve and its error, and designs for segregating nonrandom variation. Discussions focus on replacement of missing values, randomized groups, calculation of the line, analysis of the variation about the line, comparative biological assays, analytical biological assays, and determination of activity. The text then ponders on measurement of relative potency and correction of quantitative variables. The manuscript takes a look at assays where the variation in response is a function of the dose and slope-ratio assays. Topics include microbiological assays and the slope-ratio technique, analysis of balanced slope-ratio assays, analysis of assays with an all-or-none response, and graded response with unequal variance. The publication then tackles multiple or repeated assays, including quality control in repeated assays and combination of independent assays of a single unknown. The publication is a valuable source of information for researchers interested in the biological assay of vitamins.

Author : Shiqi Peng
Publisher : John Wiley & Sons
Page : 525 pages
File Size : 28,70 MB
Release : 2009-11-09
Category : Medical
ISBN : 0470227605

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The definitive compendium of bioassay procedures and applications A virtual encyclopedia of key bioassay protocols, this up-to-date, essential resource reviews the methods and applications of bioassays that quantify drug activity and evaluate the validity of pharmacological models. Demonstrating the specific ways in which various pharmaceutical bioassays interpret the activity of drug molecules, the book covers the evaluation and screening of drug compounds in a wide spectrum of therapeutic categories. Throughout, the authors use various models to link experimental observations with findings that clarify the effect of drug compounds on cellular biology and measure the pharmacological activity of chemical substances. This provides an important technological platform for successful drug research and clinical therapy, making Pharmaceutical Bioassays an ideal sourcebook for pharmacologists, pharmaceutical and medical researchers, analytical chemists, toxicologists, and students. Just as a fundamental knowledge of pharmaceutical bioassays is vital to understanding how bioactive agents and new drugs are defined, discovered, and developed, so too is this indispensable reference, which: Offers a complete reference on pharmaceutical bioassays and their specific applications in the assessment of therapies for cancer, Alzheimer’s, diabetes, epilepsy, inflammation, antimalarials, pharmacogenomics, and other major therapeutic areas Covers bioassays that measure toxicity, including those for chemical toxicity and hepatoxicity—a particularly significant consideration in the development of safe and tolerable medications Highlights clinically relevant methods, ideas, and techniques to support any ­pharmaceutical researcher Suggests guidelines and systems to enhance communication between ­pharmacologists, chemists, and biologists Provides an informative analysis of pharmaceutical bioassay technologies and lab applications

Author : Lars Bohlin
Publisher : Springer Science & Business Media
Page : 203 pages
File Size : 33,29 MB
Release : 2012-12-06
Category : Science
ISBN : 9401148104

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Bioassay Methods in Natural Product Research and Drug Development contains the proceedings from the Phytochemical Society of Europe's very successful symposium on this topic, held August 24-27, 1997 in Uppsala, Sweden. In this volume, leading academic and industrial scientists discuss novel methods for assaying natural products to find new structure-activity relationships. Of key importance in this process is the availability and reliability of specific bioassay methods, but chapters also discuss chemical and biological diversity and how to dereplicate natural product extracts to increase efficiency in lead discovery. Anti-tumor, HIV-inhibitory, antiprotozoal, anti-infective and immunomodulatory natural products are discussed. Various industrial projects are presented for the first time. This volume bridges the gap between academic and industrial research and scientists, and should be required reading in drug companies and faculties of pharmacy, as well as serving scientists in pharmacognosy, pharmacology, phytochemistry, natural products and drug discovery.

Author : Atta-ur-Rahman
Publisher : CRC Press
Page : 214 pages
File Size : 50,63 MB
Release : 2001-10-04
Category : Medical
ISBN : 1135287228

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The goal of an activity-directed isolation process is to isolate bioactive compounds which may provide structural leads of therapeutic importance. Whereas the traditional process of drug development is long and expensive, simple and rapid bioassays can serve as the starting point for drug discovery. This book presents a range of "bench top" bioassa

Author : Dewan S. Bhakuni
Publisher : Springer Science & Business Media
Page : 397 pages
File Size : 11,33 MB
Release : 2006-06-30
Category : Science
ISBN : 1402034849

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Bioactive Marine Natural Products is the first book available that covers all aspects of bioactive marine natural products. It fills the void in the literature for bioactive marine natural products. The book covers various aspects of marine natural products and it is hoped that all the major classes of bioactive compounds are included. Different classes of marine organisms and the separation and isolation techniques are discussed. The chemistry and biology of marine toxins, peptides, alkaloids, nucleosides and prostanoids are discussed in detail. Biological, toxicological and clinical evaluations are also dealt with to ensure that the book may be adopted at any stage by any practicing organic chemist or biologist, working in academia or in R and D divisions of pharmaceutical companies. Each chapter in the book includes an abstract to highlight the major points discussed in the text and concluding remarks are given. References to books, monographs, review articles and original papers are provided at the end of each chapter.

Author : Robert A. Copeland
Publisher : John Wiley & Sons
Page : 295 pages
File Size : 23,61 MB
Release : 2005-04-01
Category : Science
ISBN : 0471723266

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Vital information for discovering and optimizing new drugs "Understanding the data and the experimental details that support it has always been at the heart of good science and the assumption challenging process that leads from good science to drug discovery. This book helps medicinal chemists and pharmacologists to do exactly that in the realm of enzyme inhibitors." -Paul S. Anderson, PhD This publication provides readers with a thorough understanding of enzyme-inhibitor evaluation to assist them in their efforts to discover and optimize novel drug therapies. Key topics such as competitive, noncompetitive, and uncompetitive inhibition, slow binding, tight binding, and the use of Hill coefficients to study reaction stoichiometry are all presented. Examples of key concepts are presented with an emphasis on clinical relevance and practical applications. Targeted to medicinal chemists and pharmacologists, Evaluation of Enzyme Inhibitors in Drug Discovery focuses on the questions that they need to address: * What opportunities for inhibitor interactions with enzyme targets arise from consideration of the catalytic reaction mechanism? * How are inhibitors evaluated for potency, selectivity, and mode of action? * What are the advantages and disadvantages of specific inhibition modalities with respect to efficacy in vivo? * What information do medicinal chemists and pharmacologists need from their biochemistry and enzymology colleagues to effectively pursue lead optimization? Beginning with a discussion of the advantages of enzymes as targets for drug discovery, the publication then explores the reaction mechanisms of enzyme catalysis and the types of interactions that can occur between enzymes and inhibitory molecules that lend themselves to therapeutic use. Next are discussions of mechanistic issues that must be considered when designing enzyme assays for compound library screening and for lead optimization efforts. Finally, the publication delves into special forms of inhibition that are commonly encountered in drug discovery efforts, but can be easily overlooked or misinterpreted. This publication is designed to provide students with a solid foundation in enzymology and its role in drug discovery. Medicinal chemists and pharmacologists can refer to individual chapters as specific issues arise during the course of their ongoing drug discovery efforts.